Generally 10% neutral buffered formalin.
- See also: General notes on fixation
- Measure length, as well as the transverse and sagittal diameter of the ectocervical surface.
- Optionally, weight the sample.
- Note the symmetry of the sample, and the position of the cervical canal.
- Note whether the circumference is complete. If not, and the directions are indicated on the cone, determine the approximate position of the defect.
- Cones excised by knife should be inked on the excision surfaces.[notes 2] Those excised by laser do not need inking.
Selection and trimming
- If the cone is more than 1 cm long, take transverse slices from the top of the cone and towards the ectocervix, and stop when approximately 1 cm of the ectocervical portion of the cone remains.
- Cut the portion into radial or sagittal slices. Sagittal slices are made perpendicularly to the portion surface, and should be divided into at least the four quadrants.[notes 3]
In cases where the cone is small and fragmented, try to orient the preparations and divide them if possible to obtain sagittal slices.
- See also: General notes on gross processing
Look for dysplasia in the transformation zone.
|Histology Grade||Corresponding Cytology||Description||Image|
|CIN 1 (Grade I)||Low-grade squamous intraepithelial lesion (LSIL)||
|CIN 2/3||High-grade squamous intraepithelial lesion (HSIL)[notes 4]||
|CIN 2 (Grade II)[notes 5]||
|CIN 3 (Grade III)[notes 6]||
If a neoplasia is found, the report should include:
- The histolopathological type and degree of differentiation
- Location and extent
- See also: General notes on reporting
- For a full list of contributors, see article history. Creators of images are attributed at the image description pages, seen by clicking on the images. See Patholines:Authorship for details.
- Inking can be done with India ink, and will specify the serosa or resection margin in later histopathologic evaluation.
- Each slice may be individually numbered.
- HSIL+ encompasses HSIL, AGC, and cancer
- CIN 2+ encompasses CIN 2, CIN 3, adenocarcinoma in situ (AIS), and cancer
- CIN 3+ encompasses CIN 3, AIS, and cancer
- Monica Dahlgren, Janne Malina, Anna Måsbäck, Otto Ljungberg. Stora utskärningen. KVAST (Swedish Society of Pathology). Retrieved on 2019-09-26.