Difference between revisions of "Gross processing"

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*Generelly dissect and inspect the '''entire''' specimen, while keeping relevant parts intact enough for presentation to seniors and/or maintaining orientation.
 
*Generelly dissect and inspect the '''entire''' specimen, while keeping relevant parts intact enough for presentation to seniors and/or maintaining orientation.
 
*'''Trim''' tissues for microscopy examination to a thickness of maximum 3-4 mm.<ref group=notes>Thicker slices may not become adequately fixated in formalin.</ref>
 
*'''Trim''' tissues for microscopy examination to a thickness of maximum 3-4 mm.<ref group=notes>Thicker slices may not become adequately fixated in formalin.</ref>
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==Biopsy bags==
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Put the following types of specimens in these:
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*Tiny fragments that need to be poured out from their contianers.
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*Bloody specimens such as endometrial curettages or [[products of conception]]. For products of conception, chorionic villi may otherwise contaminate other specimens.
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*Friable tissue such as urinary bladder biopsies.
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*{{Comprehensive-begin}}Any important small piece of tissue, since specimens occasionally get lost from cassettes, and the absence of a biopsy bag in the cassette of such cases points towards a mistake made at gross processing.{{Comprehensive-end}}
 
{{Bottom}}
 
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Revision as of 09:59, 12 April 2021

Author: Mikael Häggström [note 1]

General notes edit

Further reading:

Following are general notes on selection and trimming in pathology.

Comprehensiveness

On this resource, the following formatting is used for comprehensiveness:

  • Minimal depth
  • (Moderate depth)
  • ((Comprehensive))
Other legend

<< Decision needed between alternatives separated by / signs >>
{{Common findings / In case of findings}}
[[Comments]]
Link to another page

Before cutting

  • Confirm that the patient identity on the specimen container matches the identity that will be applied to the gross description and cassettes. {{If the referral or requisition form is available, confirm the patient identity on that one as well.}}
  • Generally measure estimated volume or 3 dimensions for samples greater than 0.4 cm in greatest dimension.[notes 1]
  • Generally weigh entire organs, after having any attached tissue trimmed away if feasible.
  • ((Note the color of the sample, even when unremarkable, but do not linger on deciding it.))[notes 2]
  • Generally, use inking for resection margins where cancer radicality is important. Further information: inking
  • (On fatty or greasy surfaces, apply vinegar to emulsify and remove the fat, dry the specimen and then ink. Otherwise, vinegar can be used either before or after inking to "dry" it.)
  • (Preferably photograph or make a drawing where slices have been taken.)[1]
  • Remove any surgical stitches from samples before microtomy.
  • (At least for larger samples, consider looking for medical imaging or biopsy reports in order to better guide the process.)[2]
  • Fix bone in formalin prior to decalcification. Use reminders so not to forget bone that is decalcifying.

Cutting

  • When cutting with the longer knives, try to cut in one stroke - do not use like a saw (continuous back and forth)
  • Generally, strive to make slices perpendicular to visible interfaces of relevant tissues.
  • Generelly dissect and inspect the entire specimen, while keeping relevant parts intact enough for presentation to seniors and/or maintaining orientation.
  • Trim tissues for microscopy examination to a thickness of maximum 3-4 mm.[notes 3]

Biopsy bags

Put the following types of specimens in these:

  • Tiny fragments that need to be poured out from their contianers.
  • Bloody specimens such as endometrial curettages or products of conception. For products of conception, chorionic villi may otherwise contaminate other specimens.
  • Friable tissue such as urinary bladder biopsies.
  • ((Any important small piece of tissue, since specimens occasionally get lost from cassettes, and the absence of a biopsy bag in the cassette of such cases points towards a mistake made at gross processing.))

Notes

  1. Specifying dimensions in 3 dimensions is generally a waste of time for specimens less than 0.4 cm.
  2. The color is generally of little consequence.
  3. Thicker slices may not become adequately fixated in formalin.
  1. For a full list of contributors, see article history. Creators of images are attributed at the image description pages, seen by clicking on the images. See Patholines:Authorship for details.

Main page

References

  1. Monika Roychowdhury. Grossing (histologic sampling) of breast lesions. Pathologyoutlines.com. Topic Completed: 1 August 2012. Revised: 19 September 2019
  2. . Gross Pathology Manual By The University of Chicago Department of Pathology. Updated 2-14-19 NAC.

Image sources