https://patholines.org/index.php?title=Lymph_nodes&feed=atom&action=historyLymph nodes - Revision history2024-03-28T08:56:23ZRevision history for this page on the wikiMediaWiki 1.33.0https://patholines.org/index.php?title=Lymph_nodes&diff=6036&oldid=prevMikael Häggström: /* Gross procedure */ Touch prep2022-09-01T22:28:14Z<p><span dir="auto"><span class="autocomment">Gross procedure: </span> Touch prep</span></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 22:28, 1 September 2022</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l20" >Line 20:</td>
<td colspan="2" class="diff-lineno">Line 20:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*'''Section''' lymph nodes if needed. Lymph nodes less than 5 mm may be submitted whole, while larger lymph nodes may be sectioned at 2-3 mm intervals.<ref>{{cite web|url=https://documents.cap.org/protocols/cp-skin-melanoma-biopsy-19-4100.pdf|title=Protocol for the Examination of Biopsy Specimens From Patients With Melanoma of the Skin|website=College of American Pathologists}} Version: Melanoma Biopsy 4.1.0.0 Protocol Posting Date: August 2019</ref></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*'''Section''' lymph nodes if needed. Lymph nodes less than 5 mm may be submitted whole, while larger lymph nodes may be sectioned at 2-3 mm intervals.<ref>{{cite web|url=https://documents.cap.org/protocols/cp-skin-melanoma-biopsy-19-4100.pdf|title=Protocol for the Examination of Biopsy Specimens From Patients With Melanoma of the Skin|website=College of American Pathologists}} Version: Melanoma Biopsy 4.1.0.0 Protocol Posting Date: August 2019</ref></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Generally do not submit multiple sectioned lymph nodes in the same '''cassette''', to allow exact counting of the number of involved lymph nodes on microscopy. If you will nevertheless submit multiple bisected lymph nodes in the same cassette, '''[[ink]]''' each lymph node differently.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Generally do not submit multiple sectioned lymph nodes in the same '''cassette''', to allow exact counting of the number of involved lymph nodes on microscopy. If you will nevertheless submit multiple bisected lymph nodes in the same cassette, '''[[ink]]''' each lymph node differently.</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>*If suspected '''[[lymphoma]]''', such as an enlarged lymph node without any adjacent tumor or another almost certain cause, make a touch prep <del class="diffchange diffchange-inline">(press a glass slide against the cut surface of the lymph node, apply cytologic fixative solution immediately and stain with H&E)</del>. Also, take a small fresh sample for flow cytometry: </div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins class="diffchange diffchange-inline">[[File:Touch prep on a lymph node.jpg|thumb|220px|Making a "touch prep": Press a glass slide against the cut surface of the lymph node, apply cytologic fixative solution immediately and stain with H&E.]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>*If suspected '''[[lymphoma]]''', such as an enlarged lymph node without any adjacent tumor or another almost certain cause, make a touch prep. Also, take a small fresh sample for flow cytometry: </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:*For '''flow cytometry''', aim for a tissue size of approximately 5 mm<sup>3</sup>. Put it in specific flow cytometry preservative medium (such as RPMI), and ensure it gets to the flow cytometry lab. If it is after normal hours and there is no one to ask to find such medium, you can put the specimen in normal sterile saline (enough to cover the tissue) in a fridge (2-8°C) until the next morning.<ref>{{cite web|url=https://lifelabs.azureedge.net/lifelabs-wp-cdn/wp-content/uploads/2018/08/SPECIMEN-INFORMATION-AND-REQUIREMENTS-FOR-FLOW-CYTOMETRY-TESTING.pdf|title=Specimen Information and Requirements for Flow Cytometry Testing|website=Lifelabs}} Doc #8218 Ver: 7.0 Current Issued: 13-Apr-2018</ref> If you receive multiple lymph nodes for flow cytometry, still only sample one (unless the referral asks for separate flow cytometry studies, or there is a given history of one lymph node having high uptake and another having low uptake on PET scanning).</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:*For '''flow cytometry''', aim for a tissue size of approximately 5 mm<sup>3</sup>. Put it in specific flow cytometry preservative medium (such as RPMI), and ensure it gets to the flow cytometry lab. If it is after normal hours and there is no one to ask to find such medium, you can put the specimen in normal sterile saline (enough to cover the tissue) in a fridge (2-8°C) until the next morning.<ref>{{cite web|url=https://lifelabs.azureedge.net/lifelabs-wp-cdn/wp-content/uploads/2018/08/SPECIMEN-INFORMATION-AND-REQUIREMENTS-FOR-FLOW-CYTOMETRY-TESTING.pdf|title=Specimen Information and Requirements for Flow Cytometry Testing|website=Lifelabs}} Doc #8218 Ver: 7.0 Current Issued: 13-Apr-2018</ref> If you receive multiple lymph nodes for flow cytometry, still only sample one (unless the referral asks for separate flow cytometry studies, or there is a given history of one lymph node having high uptake and another having low uptake on PET scanning).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
</table>Mikael Häggströmhttps://patholines.org/index.php?title=Lymph_nodes&diff=5792&oldid=prevMikael Häggström: Integrated2022-06-22T16:17:13Z<p>Integrated</p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 16:17, 22 June 2022</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l109" >Line 109:</td>
<td colspan="2" class="diff-lineno">Line 109:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:*'''Enlargement''', as preferably measured during grossing, but can possibly be made on the microscopy slide. If present, see separate section below.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:*'''Enlargement''', as preferably measured during grossing, but can possibly be made on the microscopy slide. If present, see separate section below.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><gallery mode=packed heights=200></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><gallery mode=packed heights=200></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>File:Microscopic lymph node screening.jpg|'''Metastases''': generally first look around the edges with intermediate magnification, and low mag in the middle, since cancer metastases usually occur at edges (as in this case).<del class="diffchange diffchange-inline"><ref group=notes>Urothelial </del>cancers, however, have a tendency to show up anywhere in lymph nodes.<del class="diffchange diffchange-inline"></ref></del></div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>File:Microscopic lymph node screening.jpg|'''Metastases''': generally first look around the edges with intermediate magnification, and low mag in the middle, since cancer metastases usually occur at edges (as in this case). <ins class="diffchange diffchange-inline">For suspected urothelial </ins>cancers, however, <ins class="diffchange diffchange-inline">look closely throughout the node, as they </ins>have a tendency to show up anywhere in lymph nodes.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>File:Lymph node metastasis from neuroendocrine tumor.jpg|Lymph node '''metastasis''' from a [[neuroendocrine tumor of the midgut]]. Metastates generally look similar to its primary tumor.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>File:Lymph node metastasis from neuroendocrine tumor.jpg|Lymph node '''metastasis''' from a [[neuroendocrine tumor of the midgut]]. Metastates generally look similar to its primary tumor.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>File:Granuloma 20x.jpg|'''Granulomas''' (non-necrotizing granuloma pictured). If seen generally perform staining for acid-fast bacilli, and GMS stain for fungi.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>File:Granuloma 20x.jpg|'''Granulomas''' (non-necrotizing granuloma pictured). If seen generally perform staining for acid-fast bacilli, and GMS stain for fungi.</div></td></tr>
</table>Mikael Häggströmhttps://patholines.org/index.php?title=Lymph_nodes&diff=5789&oldid=prevMikael Häggström: /* Defining a lymph node */ Integrated2022-06-22T16:12:34Z<p><span dir="auto"><span class="autocomment">Defining a lymph node: </span> Integrated</span></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 16:12, 22 June 2022</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l102" >Line 102:</td>
<td colspan="2" class="diff-lineno">Line 102:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Microscopic examination==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Microscopic examination==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Defining a lymph node===</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Defining a lymph node===</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>For counting lymph nodes, each should have a discernible capsule around lymphoid cells. Also count larger free-standing lymphoid aggregates.<del class="diffchange diffchange-inline"><ref group=notes></del>However, the definition of what constitutes a lymph node is largely subjective.<<del class="diffchange diffchange-inline">br</del>> <del class="diffchange diffchange-inline">-</del>{{cite journal| author=Parkash V, Bifulco C, Feinn R, Concato J, Jain D| title=To count and how to count, that is the question: interobserver and intraobserver variability among pathologists in lymph node counting. | journal=Am J Clin Pathol | year= 2010 | volume= 134 | issue= 1 | pages= 42-9 | pmid=20551265 | doi=10.1309/AJCPO92DZMUCGEUF | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=20551265 }}</ref> Also strive to keep a consistency with the gross description. In addition, any cancer involvement is in itself a relative indication of being a lymph node.</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>For counting lymph nodes, each should have a discernible capsule around lymphoid cells. Also count larger free-standing lymphoid aggregates. However, the definition of what constitutes a lymph node is largely subjective.<<ins class="diffchange diffchange-inline">ref</ins>>{{cite journal| author=Parkash V, Bifulco C, Feinn R, Concato J, Jain D| title=To count and how to count, that is the question: interobserver and intraobserver variability among pathologists in lymph node counting. | journal=Am J Clin Pathol | year= 2010 | volume= 134 | issue= 1 | pages= 42-9 | pmid=20551265 | doi=10.1309/AJCPO92DZMUCGEUF | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=20551265 }}</ref> Also strive to keep a consistency with the gross description. In addition, any cancer involvement is in itself a relative indication of being a lymph node.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===General screening===</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===General screening===</div></td></tr>
</table>Mikael Häggströmhttps://patholines.org/index.php?title=Lymph_nodes&diff=5788&oldid=prevMikael Häggström: /* Gross processing */ note2022-06-22T13:00:34Z<p><span dir="auto"><span class="autocomment">Gross processing: </span> note</span></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 13:00, 22 June 2022</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l9" >Line 9:</td>
<td colspan="2" class="diff-lineno">Line 9:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>In samples with '''tumors''', slice through all included fat while palpating and looking for lymph nodes, and submit all that are found.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>In samples with '''tumors''', slice through all included fat while palpating and looking for lymph nodes, and submit all that are found.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>For lymph nodes taken for potential '''breast cancer''' metastasis, find out and report the procurement time and the time when put in formalin.<ref group=<del class="diffchange diffchange-inline">notes</del>>The duration that a specimen has been without formalin affects mainly the reliability of estreogen and progesteron receptor testing:<br>- {{cite journal|last1=Pekmezci|first1=Melike|last2=Szpaderska|first2=Anna|last3=Osipo|first3=Clodia|last4=Erşahin|first4=Çağatay|title=The Effect of Cold Ischemia Time and/or Formalin Fixation on Estrogen Receptor, Progesterone Receptor, and Human Epidermal Growth Factor Receptor-2 Results in Breast Carcinoma|journal=Pathology Research International|volume=2012|year=2012|pages=1–7|issn=2090-8091|doi=10.1155/2012/947041}}</ref></div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>For lymph nodes taken for potential '''breast cancer''' metastasis, find out and report the procurement time and the time when put in formalin.<ref group=<ins class="diffchange diffchange-inline">note</ins>>The duration that a specimen has been without formalin affects mainly the reliability of estreogen and progesteron receptor testing:<br>- {{cite journal|last1=Pekmezci|first1=Melike|last2=Szpaderska|first2=Anna|last3=Osipo|first3=Clodia|last4=Erşahin|first4=Çağatay|title=The Effect of Cold Ischemia Time and/or Formalin Fixation on Estrogen Receptor, Progesterone Receptor, and Human Epidermal Growth Factor Receptor-2 Results in Breast Carcinoma|journal=Pathology Research International|volume=2012|year=2012|pages=1–7|issn=2090-8091|doi=10.1155/2012/947041}}</ref></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Gross procedure===</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Gross procedure===</div></td></tr>
</table>Mikael Häggströmhttps://patholines.org/index.php?title=Lymph_nodes&diff=4876&oldid=prevMikael Häggström: Not necessary for non-sentinel2022-03-01T16:00:32Z<p>Not necessary for non-sentinel</p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 16:00, 1 March 2022</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l76" >Line 76:</td>
<td colspan="2" class="diff-lineno">Line 76:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Urgency===</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Urgency===</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>The processing of lymph nodes <del class="diffchange diffchange-inline">may be </del>rushed when the H&E stain will determine whether immunohistochemistry will be performed, especially when a lymph node is submitted together with a separate specimen that may be solved without immunostains. This rushing allows you to have the immunostained slides by a similar time as the rest of the case.<ref name="pmid12610108">{{cite journal| author=Chandler IP, Oommen R, Lawson CW| title=Invasive lobular carcinoma and cytokeratin immunohistochemistry: an audit. | journal=J Clin Pathol | year= 2003 | volume= 56 | issue= 3 | pages= 240 | pmid=12610108 | doi=10.1136/jcp.56.3.240 | pmc=1769908 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=12610108 }} </ref> Examples of cases that <del class="diffchange diffchange-inline">may be </del>rushed for such reasons include those that may be stained by CK AE1/AE3 in order to visualize otherwise occult lymph node involvement if you don't see any involvement on the H&E stain, mainly in cases when one or more sentinel lymph nodes are submitted together with any of the following:</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>The processing of lymph nodes <ins class="diffchange diffchange-inline">is preferably </ins>rushed when the H&E stain will determine whether immunohistochemistry will be performed, especially when a lymph node is submitted together with a separate specimen that may be solved without immunostains. This rushing allows you to have the immunostained slides by a similar time as the rest of the case.<ref name="pmid12610108">{{cite journal| author=Chandler IP, Oommen R, Lawson CW| title=Invasive lobular carcinoma and cytokeratin immunohistochemistry: an audit. | journal=J Clin Pathol | year= 2003 | volume= 56 | issue= 3 | pages= 240 | pmid=12610108 | doi=10.1136/jcp.56.3.240 | pmc=1769908 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=12610108 }} </ref> Examples of cases that <ins class="diffchange diffchange-inline">are preferably </ins>rushed for such reasons include those that may be stained by CK AE1/AE3 in order to visualize otherwise occult lymph node involvement if you don't see any involvement on the H&E stain, mainly in cases when one or more sentinel lymph nodes are submitted together with any of the following:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*A [[breast biopsy or excision]] of a suspected or previously confirmed [[invasive lobular carcinoma]] (but not necessarily invasive carcinoma with lobular features)</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*A [[breast biopsy or excision]] of a suspected or previously confirmed [[invasive lobular carcinoma]] (but not necessarily invasive carcinoma with lobular features)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*A [[uterus]] specimen of a suspected or previously confirmed [[endometrial cancer]].</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*A [[uterus]] specimen of a suspected or previously confirmed [[endometrial cancer]].</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Rushing is not necessary for non-sentinel lymph nodes.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Gross report===</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Gross report===</div></td></tr>
</table>Mikael Häggströmhttps://patholines.org/index.php?title=Lymph_nodes&diff=4868&oldid=prevMikael Häggström: /* Gross processing */ +Urgency2022-02-24T15:18:18Z<p><span dir="auto"><span class="autocomment">Gross processing: </span> +Urgency</span></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 15:18, 24 February 2022</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l74" >Line 74:</td>
<td colspan="2" class="diff-lineno">Line 74:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Lymphadenopathy of more than 1.5&nbsp;cm - 2&nbsp;cm increases the risk of cancer or granulomatous disease as the cause rather than only inflammation or infection.<ref name="pmid12484692">{{cite journal | vauthors = Bazemore AW, Smucker DR | title = Lymphadenopathy and malignancy | journal = American Family Physician | volume = 66 | issue = 11 | pages = 2103–10 | date = December 2002 | pmid = 12484692 }}</ref></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Lymphadenopathy of more than 1.5&nbsp;cm - 2&nbsp;cm increases the risk of cancer or granulomatous disease as the cause rather than only inflammation or infection.<ref name="pmid12484692">{{cite journal | vauthors = Bazemore AW, Smucker DR | title = Lymphadenopathy and malignancy | journal = American Family Physician | volume = 66 | issue = 11 | pages = 2103–10 | date = December 2002 | pmid = 12484692 }}</ref></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">===Urgency===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">The processing of lymph nodes may be rushed when the H&E stain will determine whether immunohistochemistry will be performed, especially when a lymph node is submitted together with a separate specimen that may be solved without immunostains. This rushing allows you to have the immunostained slides by a similar time as the rest of the case.<ref name="pmid12610108">{{cite journal| author=Chandler IP, Oommen R, Lawson CW| title=Invasive lobular carcinoma and cytokeratin immunohistochemistry: an audit. | journal=J Clin Pathol | year= 2003 | volume= 56 | issue= 3 | pages= 240 | pmid=12610108 | doi=10.1136/jcp.56.3.240 | pmc=1769908 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=12610108 }} </ref> Examples of cases that may be rushed for such reasons include those that may be stained by CK AE1/AE3 in order to visualize otherwise occult lymph node involvement if you don't see any involvement on the H&E stain, mainly in cases when one or more sentinel lymph nodes are submitted together with any of the following:</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">*A [[breast biopsy or excision]] of a suspected or previously confirmed [[invasive lobular carcinoma]] (but not necessarily invasive carcinoma with lobular features)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">*A [[uterus]] specimen of a suspected or previously confirmed [[endometrial cancer]].</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Gross report===</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Gross report===</div></td></tr>
</table>Mikael Häggströmhttps://patholines.org/index.php?title=Lymph_nodes&diff=4253&oldid=prevMikael Häggström: /* General screening */ +Workup2021-11-22T19:15:08Z<p><span dir="auto"><span class="autocomment">General screening: </span> +Workup</span></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 19:15, 22 November 2021</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l101" >Line 101:</td>
<td colspan="2" class="diff-lineno">Line 101:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Look for:</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Look for:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Whatever pathology is '''indicated''' by the referral, or findings in other submitted specimens.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Whatever pathology is '''indicated''' by the referral, or findings in other submitted specimens.</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del class="diffchange diffchange-inline">*General '''screening''':</del></div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:*'''Enlargement''', as preferably measured during grossing, but can possibly be made on the microscopy slide. If present, see <ins class="diffchange diffchange-inline">separate </ins>section below<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del class="diffchange diffchange-inline">:*Any '''metastasis''', which usually looks similar to the primary tumor (neuroendocrine tumor in picture at right).</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:*'''Enlargement''', as preferably measured during grossing, but can possibly be made on the microscopy slide. If present, see section below<del class="diffchange diffchange-inline">:</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div> </div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><gallery mode=packed heights=200></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><gallery mode=packed heights=200></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>File:Microscopic lymph node screening.jpg|<del class="diffchange diffchange-inline">When looking for </del>'''<del class="diffchange diffchange-inline">metastases</del>'''<del class="diffchange diffchange-inline">, </del>generally first look around the edges with intermediate magnification, and low mag in the middle, since cancer metastases usually occur at edges (as in this case).<ref group=notes>Urothelial cancers, however, have a tendency to show up anywhere in lymph nodes.</ref></div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>File:Microscopic lymph node screening.jpg|'''<ins class="diffchange diffchange-inline">Metastases</ins>'''<ins class="diffchange diffchange-inline">: </ins>generally first look around the edges with intermediate magnification, and low mag in the middle, since cancer metastases usually occur at edges (as in this case).<ref group=notes>Urothelial cancers, however, have a tendency to show up anywhere in lymph nodes.</ref></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>File:Lymph node metastasis from neuroendocrine tumor.jpg|Lymph node metastasis from a [[neuroendocrine tumor of the midgut]].</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>File:Lymph node metastasis from neuroendocrine tumor.jpg|Lymph node <ins class="diffchange diffchange-inline">'''</ins>metastasis<ins class="diffchange diffchange-inline">''' </ins>from a [[neuroendocrine tumor of the midgut]]<ins class="diffchange diffchange-inline">. Metastates generally look similar to its primary tumor.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins class="diffchange diffchange-inline">File:Granuloma 20x.jpg|'''Granulomas''' (non-necrotizing granuloma pictured). If seen generally perform staining for acid-fast bacilli, and GMS stain for fungi</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div></gallery></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div></gallery></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
</table>Mikael Häggströmhttps://patholines.org/index.php?title=Lymph_nodes&diff=4250&oldid=prevMikael Häggström: /* Gross procedure */ °2021-11-22T00:43:13Z<p><span dir="auto"><span class="autocomment">Gross procedure: </span> °</span></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 00:43, 22 November 2021</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l21" >Line 21:</td>
<td colspan="2" class="diff-lineno">Line 21:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Generally do not submit multiple sectioned lymph nodes in the same '''cassette''', to allow exact counting of the number of involved lymph nodes on microscopy. If you will nevertheless submit multiple bisected lymph nodes in the same cassette, '''[[ink]]''' each lymph node differently.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Generally do not submit multiple sectioned lymph nodes in the same '''cassette''', to allow exact counting of the number of involved lymph nodes on microscopy. If you will nevertheless submit multiple bisected lymph nodes in the same cassette, '''[[ink]]''' each lymph node differently.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*If suspected '''[[lymphoma]]''', such as an enlarged lymph node without any adjacent tumor or another almost certain cause, make a touch prep (press a glass slide against the cut surface of the lymph node, apply cytologic fixative solution immediately and stain with H&E). Also, take a small fresh sample for flow cytometry: </div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*If suspected '''[[lymphoma]]''', such as an enlarged lymph node without any adjacent tumor or another almost certain cause, make a touch prep (press a glass slide against the cut surface of the lymph node, apply cytologic fixative solution immediately and stain with H&E). Also, take a small fresh sample for flow cytometry: </div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:*For '''flow cytometry''', aim for a tissue size of approximately 5 mm<sup>3</sup>. Put it in specific flow cytometry preservative medium (such as RPMI), and ensure it gets to the flow cytometry lab. If it is after normal hours and there is no one to ask to find such medium, you can put the specimen in normal sterile saline (enough to cover the tissue) in a fridge (2-<del class="diffchange diffchange-inline">8<sup>o</sup>C</del>) until the next morning.<ref>{{cite web|url=https://lifelabs.azureedge.net/lifelabs-wp-cdn/wp-content/uploads/2018/08/SPECIMEN-INFORMATION-AND-REQUIREMENTS-FOR-FLOW-CYTOMETRY-TESTING.pdf|title=Specimen Information and Requirements for Flow Cytometry Testing|website=Lifelabs}} Doc #8218 Ver: 7.0 Current Issued: 13-Apr-2018</ref> If you receive multiple lymph nodes for flow cytometry, still only sample one (unless the referral asks for separate flow cytometry studies, or there is a given history of one lymph node having high uptake and another having low uptake on PET scanning).</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:*For '''flow cytometry''', aim for a tissue size of approximately 5 mm<sup>3</sup>. Put it in specific flow cytometry preservative medium (such as RPMI), and ensure it gets to the flow cytometry lab. If it is after normal hours and there is no one to ask to find such medium, you can put the specimen in normal sterile saline (enough to cover the tissue) in a fridge (2-<ins class="diffchange diffchange-inline">8°C</ins>) until the next morning.<ref>{{cite web|url=https://lifelabs.azureedge.net/lifelabs-wp-cdn/wp-content/uploads/2018/08/SPECIMEN-INFORMATION-AND-REQUIREMENTS-FOR-FLOW-CYTOMETRY-TESTING.pdf|title=Specimen Information and Requirements for Flow Cytometry Testing|website=Lifelabs}} Doc #8218 Ver: 7.0 Current Issued: 13-Apr-2018</ref> If you receive multiple lymph nodes for flow cytometry, still only sample one (unless the referral asks for separate flow cytometry studies, or there is a given history of one lymph node having high uptake and another having low uptake on PET scanning).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Definition of an enlarged lymph node===</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Definition of an enlarged lymph node===</div></td></tr>
</table>Mikael Häggströmhttps://patholines.org/index.php?title=Lymph_nodes&diff=4249&oldid=prevMikael Häggström: /* Gross procedure */ +Multiple2021-11-22T00:41:56Z<p><span dir="auto"><span class="autocomment">Gross procedure: </span> +Multiple</span></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 00:41, 22 November 2021</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l21" >Line 21:</td>
<td colspan="2" class="diff-lineno">Line 21:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Generally do not submit multiple sectioned lymph nodes in the same '''cassette''', to allow exact counting of the number of involved lymph nodes on microscopy. If you will nevertheless submit multiple bisected lymph nodes in the same cassette, '''[[ink]]''' each lymph node differently.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Generally do not submit multiple sectioned lymph nodes in the same '''cassette''', to allow exact counting of the number of involved lymph nodes on microscopy. If you will nevertheless submit multiple bisected lymph nodes in the same cassette, '''[[ink]]''' each lymph node differently.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*If suspected '''[[lymphoma]]''', such as an enlarged lymph node without any adjacent tumor or another almost certain cause, make a touch prep (press a glass slide against the cut surface of the lymph node, apply cytologic fixative solution immediately and stain with H&E). Also, take a small fresh sample for flow cytometry: </div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*If suspected '''[[lymphoma]]''', such as an enlarged lymph node without any adjacent tumor or another almost certain cause, make a touch prep (press a glass slide against the cut surface of the lymph node, apply cytologic fixative solution immediately and stain with H&E). Also, take a small fresh sample for flow cytometry: </div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:*For flow cytometry, aim for a tissue size of approximately 5 mm<sup>3</sup>. Put it in specific flow cytometry preservative medium (such as RPMI), and ensure it gets to the flow cytometry lab. If it is after normal hours and there is no one to ask to find such medium, you can put the specimen in normal sterile saline (enough to cover the tissue) in a fridge (2-8<sup>o</sup>C) until the next morning.<ref>{{cite web|url=https://lifelabs.azureedge.net/lifelabs-wp-cdn/wp-content/uploads/2018/08/SPECIMEN-INFORMATION-AND-REQUIREMENTS-FOR-FLOW-CYTOMETRY-TESTING.pdf|title=Specimen Information and Requirements for Flow Cytometry Testing|website=Lifelabs}} Doc #8218 Ver: 7.0 Current Issued: 13-Apr-2018</ref></div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:*For <ins class="diffchange diffchange-inline">'''</ins>flow cytometry<ins class="diffchange diffchange-inline">'''</ins>, aim for a tissue size of approximately 5 mm<sup>3</sup>. Put it in specific flow cytometry preservative medium (such as RPMI), and ensure it gets to the flow cytometry lab. If it is after normal hours and there is no one to ask to find such medium, you can put the specimen in normal sterile saline (enough to cover the tissue) in a fridge (2-8<sup>o</sup>C) until the next morning.<ref>{{cite web|url=https://lifelabs.azureedge.net/lifelabs-wp-cdn/wp-content/uploads/2018/08/SPECIMEN-INFORMATION-AND-REQUIREMENTS-FOR-FLOW-CYTOMETRY-TESTING.pdf|title=Specimen Information and Requirements for Flow Cytometry Testing|website=Lifelabs}} Doc #8218 Ver: 7.0 Current Issued: 13-Apr-2018</ref> <ins class="diffchange diffchange-inline">If you receive multiple lymph nodes for flow cytometry, still only sample one (unless the referral asks for separate flow cytometry studies, or there is a given history of one lymph node having high uptake and another having low uptake on PET scanning).</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Definition of an enlarged lymph node===</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Definition of an enlarged lymph node===</div></td></tr>
</table>Mikael Häggströmhttps://patholines.org/index.php?title=Lymph_nodes&diff=4248&oldid=prevMikael Häggström: /* Gross procedure */ Ensure2021-11-19T22:53:20Z<p><span dir="auto"><span class="autocomment">Gross procedure: </span> Ensure</span></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 22:53, 19 November 2021</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l21" >Line 21:</td>
<td colspan="2" class="diff-lineno">Line 21:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Generally do not submit multiple sectioned lymph nodes in the same '''cassette''', to allow exact counting of the number of involved lymph nodes on microscopy. If you will nevertheless submit multiple bisected lymph nodes in the same cassette, '''[[ink]]''' each lymph node differently.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Generally do not submit multiple sectioned lymph nodes in the same '''cassette''', to allow exact counting of the number of involved lymph nodes on microscopy. If you will nevertheless submit multiple bisected lymph nodes in the same cassette, '''[[ink]]''' each lymph node differently.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*If suspected '''[[lymphoma]]''', such as an enlarged lymph node without any adjacent tumor or another almost certain cause, make a touch prep (press a glass slide against the cut surface of the lymph node, apply cytologic fixative solution immediately and stain with H&E). Also, take a small fresh sample for flow cytometry: </div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*If suspected '''[[lymphoma]]''', such as an enlarged lymph node without any adjacent tumor or another almost certain cause, make a touch prep (press a glass slide against the cut surface of the lymph node, apply cytologic fixative solution immediately and stain with H&E). Also, take a small fresh sample for flow cytometry: </div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:*For flow cytometry, aim for a tissue size of approximately 5 mm<sup>3</sup>. <del class="diffchange diffchange-inline">Use </del>specific flow cytometry preservative medium (such as RPMI). If it is after normal hours and there is no one to ask to find such medium, you can put the specimen in normal sterile saline (enough to cover the tissue) in a fridge (2-8<sup>o</sup>C) until the next morning.<ref>{{cite web|url=https://lifelabs.azureedge.net/lifelabs-wp-cdn/wp-content/uploads/2018/08/SPECIMEN-INFORMATION-AND-REQUIREMENTS-FOR-FLOW-CYTOMETRY-TESTING.pdf|title=Specimen Information and Requirements for Flow Cytometry Testing|website=Lifelabs}} Doc #8218 Ver: 7.0 Current Issued: 13-Apr-2018</ref></div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:*For flow cytometry, aim for a tissue size of approximately 5 mm<sup>3</sup>. <ins class="diffchange diffchange-inline">Put it in </ins>specific flow cytometry preservative medium (such as RPMI)<ins class="diffchange diffchange-inline">, and ensure it gets to the flow cytometry lab</ins>. If it is after normal hours and there is no one to ask to find such medium, you can put the specimen in normal sterile saline (enough to cover the tissue) in a fridge (2-8<sup>o</sup>C) until the next morning.<ref>{{cite web|url=https://lifelabs.azureedge.net/lifelabs-wp-cdn/wp-content/uploads/2018/08/SPECIMEN-INFORMATION-AND-REQUIREMENTS-FOR-FLOW-CYTOMETRY-TESTING.pdf|title=Specimen Information and Requirements for Flow Cytometry Testing|website=Lifelabs}} Doc #8218 Ver: 7.0 Current Issued: 13-Apr-2018</ref></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Definition of an enlarged lymph node===</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Definition of an enlarged lymph node===</div></td></tr>
</table>Mikael Häggström