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Note
{{Fixation - standard}}
Fresh breast specimens should be put in formalin within one hour.<ref group=notes note name=time>An increased cold ischemia time (time from procurement to time in formalin) negatively effects the utility of immunohistochemistry.<br>- {{cite journal|last1=Khoury|first1=Thaer|title=Delay to Formalin Fixation (Cold Ischemia Time) Effect on Breast Cancer Molecules|journal=American Journal of Clinical Pathology|volume=149|issue=4|year=2018|pages=275–292|issn=0002-9173|doi=10.1093/ajcp/aqx164}}</ref> Breast specimens should be immersed in formalin for 6-72 hours.<ref>{{cite web|url=https://webapps.cap.org/apps/docs/committees/immunohistochemistry/her2_faqs.pdf|title=Recommendations for HER2 Testing in Breast Cancer: ASCO – CAP Clinical Practice Guideline Update|date=2013-10-17|website=College of American Pathologists}}</ref>
{{Comprehensiveness|otherlegend=yes}}
*Determine total specimen '''size'''. {{Comprehensive-begin}}Weight the specimen<ref name=Roychowdhury>{{cite web|url=https://www.pathologyoutlines.com/topic/breastmalignantgrossing.html|title=Grossing (histologic sampling) of breast lesions|author=Monika Roychowdhury|website=Pathologyoutlines.com}} Topic Completed: 1 August 2012. Revised: 19 September 2019</ref>{{Comprehensive-end}}
*'''[[Ink]]''' margins. If sample orientations are marked, use different colors for different directions.<ref name=Roychowdhury/>
*'''Palpate''' the specimen for masses. If felt, estimate the greatest dimension.<ref group=notesnote>A palpated greatest dimension before cutting is still superior to trying to align cut pieces together or mathematically adding the thicknesses of involved slices.</ref> Compare with radiography if available.<ref name=Roychowdhury/> Confirm the presence of any known biopsy clips, either visibly or by post-operative radiography (in order to confirm that the specimen includes the region of interest).
[[File:Cutting of uneven lumpectomy.jpg|thumb|240px|Cut a lumpectomy in the direction that gives the shortest distance from margin to margin. If a lumpectomy is uneven as shown, cut so that the first and last slice become wedge shaped (because these will later be cut further, perpendicularly to the margin), avoiding a wedge-shape for remaining slices.]]
*'''Serially section''' the specimen.
:*Consistency<ref name=Roychowdhury/>
*{{Moderate-begin}}Description of sectioning and submissions.{{Moderate-end}}
*{{Comprehensive-begin}}Time of procurement and time of placement in formalin.<ref group=notes note name=time/>{{Comprehensive-end}}
Example:
|rowspan=3| '''[[Fibrocystic breast changes]]''' ||rowspan=3| 40% || '''[[Sclerosing adenosis]]''' (pictured), with an increase in glandular elements in addition to stromal proliferation that distorts and compresses glands.<ref>{{cite web|url=https://www.pathologyoutlines.com/topic/breastsclerosingadenosis.html|title=Breast - Fibrocystic changes - Sclerosing adenosis|author=Jaya Ruth Asirvatham, M.B.B.S., Julie M. Jorns, M.D.|website=Pathology Outlines}} Topic Completed: 1 January 2015. Minor changes: 31 December 2020</ref> || [[File:Histopathology of sclerosing adenosis of the breast.jpg|180px]]
|-
| '''Radial scar''', seen as a fibroelastic stroma and entrapped glands radiating outward. Measure the size of these.<ref group=notesnote>Size is a major factor in whether to fully excise radial scars, at an approximate cutoff at around 1 cm.</ref>
| [[File:Histopathology of a radial scar of the breast.jpg|180px]]
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| No disease || 30% || ||
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| [[Fibroepithelial tumor]] || 7% || Proliferation of both stromal and epithelial components.<ref group="notes"note>The proliferation of two histological components is called "biplasia", from ''Latin'' bis (“twice”) and -plasia (“formation”), or "biphasic proliferation"</ref> The tumor group mainly includes fibroadenoma and phyllodes tumor.
| [[File:Micrograph of a fibroadenoma.jpg|180px]]
|-
