Immunofluorescense of skin tissues - Revision history

Mikael Häggström: Bolded

2019-09-17T09:42:54Z

Bolded

← Older revision		Revision as of 05:42, 17 September 2019
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	Suspicion of one or several '''immunologic diseases''':<ref name="ChhabraMinz2012">Page 678 in: {{cite journal\|last1=Chhabra\|first1=Seema\|last2=Minz\|first2=RanjanaWalker\|last3=Saikia\|first3=Biman\|title=Immunofluorescence in dermatology\|journal=Indian Journal of Dermatology, Venereology, and Leprology\|volume=78\|issue=6\|year=2012\|pages=677\|issn=0378-6323\|doi=10.4103/0378-6323.102355\|url=https://pdfs.semanticscholar.org/23d0/148dbe342a93e0bc65a6a2e31040a89113ba.pdf}}</ref>		Suspicion of one or several '''immunologic diseases''':<ref name="ChhabraMinz2012">Page 678 in: {{cite journal\|last1=Chhabra\|first1=Seema\|last2=Minz\|first2=RanjanaWalker\|last3=Saikia\|first3=Biman\|title=Immunofluorescence in dermatology\|journal=Indian Journal of Dermatology, Venereology, and Leprology\|volume=78\|issue=6\|year=2012\|pages=677\|issn=0378-6323\|doi=10.4103/0378-6323.102355\|url=https://pdfs.semanticscholar.org/23d0/148dbe342a93e0bc65a6a2e31040a89113ba.pdf}}</ref>

	Suspected conditions where the direct immunofluorescence (DIF) is diagnostic:<ref name="ChhabraMinz2012"/>		Suspected conditions where the '''direct immunofluorescence (DIF) is diagnostic''':<ref name="ChhabraMinz2012"/>
	*Bullous diseases		*Bullous diseases
	:*Pemphigus (all forms)		:*Pemphigus (all forms)
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	:*Systemic lupus erythematosus		:*Systemic lupus erythematosus

	Suspected conditions where DIF is highly characteristic and of some value:<ref name="ChhabraMinz2012"/>		===Highly characteristic===
			Suspected conditions where '''DIF is highly characteristic''' and of some value:<ref name="ChhabraMinz2012"/>
	*Vascular diseases		*Vascular diseases
	:*Allergic vasculitis		:*Allergic vasculitis
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	*Lichen planus		*Lichen planus

	Conditions where DIF is not diagnostic but only suggestive of immunologic pathogenesis:<ref name="ChhabraMinz2012"/>		Conditions where '''DIF is not diagnostic but only suggestive''' of immunologic pathogenesis:<ref name="ChhabraMinz2012"/>
	*Connective tissue diseases		*Connective tissue diseases
	:*Mixed connective tissue diseases		:*Mixed connective tissue diseases
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	:*Psoriasis		:*Psoriasis

	Suspected conditions where indirect immunofluorescence is generally indicated:<ref name="ChhabraMinz2012"/>		Suspected conditions where '''indirect immunofluorescence is generally indicated''':<ref name="ChhabraMinz2012"/>
	*Pemphigus vulgaris		*Pemphigus vulgaris
	*Pemphigus foliaceus		*Pemphigus foliaceus

Mikael Häggström: +Composition

2019-09-17T09:41:27Z

+Composition

← Older revision		Revision as of 05:41, 17 September 2019
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	==Indications==		==Indications==
	Suspicion of one or several '''immunologic diseases''':<ref name="ChhabraMinz2012">Page 678 in: {{cite journal\|last1=Chhabra\|first1=Seema\|last2=Minz\|first2=RanjanaWalker\|last3=Saikia\|first3=Biman\|title=Immunofluorescence in dermatology\|journal=Indian Journal of Dermatology, Venereology, and Leprology\|volume=78\|issue=6\|year=2012\|pages=677\|issn=0378-6323\|doi=10.4103/0378-6323.102355\|url=https://pdfs.semanticscholar.org/23d0/148dbe342a93e0bc65a6a2e31040a89113ba.pdf}}</ref> Fixation for immunofluorescence, with for example [[Michel's solution]].<ref name=kvast>{{cite web\|url=http://www.svfp.se/foreningar/uploads/L15178/kvast/hud/Handlaggning%20av%20hudprover%20%20provtagningsanvisningar%20utskarningsprinciper%20och%20snittning%2020150325.pdf\|title=Handläggning av hudprover – provtagningsanvisningar, utskärningsprinciper och snittning (Handling of skin samples - Instructions for sampling, cutting and incision\|author=Katarzyna Lundmark, Krynitz, Ismini Vassilaki, Lena Mölne, Annika Ternesten Bratel\|accessdate=2019-09-09\|website=KVAST (Swedish Society of Pathology)}}</ref>		Suspicion of one or several '''immunologic diseases''':<ref name="ChhabraMinz2012">Page 678 in: {{cite journal\|last1=Chhabra\|first1=Seema\|last2=Minz\|first2=RanjanaWalker\|last3=Saikia\|first3=Biman\|title=Immunofluorescence in dermatology\|journal=Indian Journal of Dermatology, Venereology, and Leprology\|volume=78\|issue=6\|year=2012\|pages=677\|issn=0378-6323\|doi=10.4103/0378-6323.102355\|url=https://pdfs.semanticscholar.org/23d0/148dbe342a93e0bc65a6a2e31040a89113ba.pdf}}</ref>

	Suspected conditions where the direct immunofluorescence (DIF) is diagnostic:<ref name="ChhabraMinz2012"/>		Suspected conditions where the direct immunofluorescence (DIF) is diagnostic:<ref name="ChhabraMinz2012"/>
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	*ANCA associated SVV		*ANCA associated SVV

			==Fixation==
			For example ''Michel's solution'',<ref name=kvast>{{cite web\|url=http://www.svfp.se/foreningar/uploads/L15178/kvast/hud/Handlaggning%20av%20hudprover%20%20provtagningsanvisningar%20utskarningsprinciper%20och%20snittning%2020150325.pdf\|title=Handläggning av hudprover – provtagningsanvisningar, utskärningsprinciper och snittning (Handling of skin samples - Instructions for sampling, cutting and incision\|author=Katarzyna Lundmark, Krynitz, Ismini Vassilaki, Lena Mölne, Annika Ternesten Bratel\|accessdate=2019-09-09\|website=KVAST (Swedish Society of Pathology)}}</ref> minimally consisting of ammonium sulfate and saline.<ref>{{cite journal\|last1=Niedecken\|first1=Heinz W.\|last2=Lange\|first2=Claus-Ekkehard\|title=Simplified fixative medium for direct immunofluorescence in skin biopsies\|journal=Journal of the American Academy of Dermatology\|volume=12\|issue=5\|year=1985\|pages=828–831\|issn=01909622\|doi=10.1016/S0190-9622(85)70103-X}}</ref>
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Mikael Häggström: +Indications

2019-09-17T09:39:30Z

+Indications

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==Indications==
Suspicion of one or several '''immunologic diseases''':<ref name="ChhabraMinz2012">Page 678 in: {{cite journal|last1=Chhabra|first1=Seema|last2=Minz|first2=RanjanaWalker|last3=Saikia|first3=Biman|title=Immunofluorescence in dermatology|journal=Indian Journal of Dermatology, Venereology, and Leprology|volume=78|issue=6|year=2012|pages=677|issn=0378-6323|doi=10.4103/0378-6323.102355|url=https://pdfs.semanticscholar.org/23d0/148dbe342a93e0bc65a6a2e31040a89113ba.pdf}}</ref> Fixation for immunofluorescence, with for example [[Michel's solution]].<ref name=kvast>{{cite web|url=http://www.svfp.se/foreningar/uploads/L15178/kvast/hud/Handlaggning%20av%20hudprover%20%20provtagningsanvisningar%20utskarningsprinciper%20och%20snittning%2020150325.pdf|title=Handläggning av hudprover – provtagningsanvisningar, utskärningsprinciper och snittning (Handling of skin samples - Instructions for sampling, cutting and incision|author=Katarzyna Lundmark, Krynitz, Ismini Vassilaki, Lena Mölne, Annika Ternesten Bratel|accessdate=2019-09-09|website=KVAST (Swedish Society of Pathology)}}</ref>

Suspected conditions where the direct immunofluorescence (DIF) is diagnostic:<ref name="ChhabraMinz2012"/>
*Bullous diseases
:*Pemphigus (all forms)
:*Pemphigoid (all forms)
:*Herpes gestationis/gestational pemphigoid
:*Dermatitis herpetiformis
:*Linear IgA bullous dermatosis Epidermolysis bullosa aquisita
*Connective tissue diseases
:*Discoid lupus erythematosus
:*Systemic lupus erythematosus

Suspected conditions where DIF is highly characteristic and of some value:<ref name="ChhabraMinz2012"/>
*Vascular diseases
:*Allergic vasculitis
:*Henoch–Schoenlein purpura
:*Essential mixed cryoglobulinemia
:*Polyarteritis nodosa
*Porphyria
:*Especially porphyria cutanea tarda
*Lichen planus

Conditions where DIF is not diagnostic but only suggestive of immunologic pathogenesis:<ref name="ChhabraMinz2012"/>
*Connective tissue diseases
:*Mixed connective tissue diseases
:*Systemic sclerosis
:*Dermatomyositis
:*Psoriasis

Suspected conditions where indirect immunofluorescence is generally indicated:<ref name="ChhabraMinz2012"/>
*Pemphigus vulgaris
*Pemphigus foliaceus
*IgA pemphigus
*Bullous pemphigoid
*Dermatitis herpetiformis
*Discoid lupus erythematosus
*Disseminated DLE
*Systemic lupus erythematosus
*Scleroderma
*Mixed connective tissue diseases
*Dermatomyositis
*Vasculitis<ref name=SLE group="notes>Sometimes used to rule out SLE by absence of anti-nuclear antibodies in uncertain cases</ref>
*Porphyria<ref name=SLE group="notes/>
*Lichen planus<ref name=SLE group="notes/>
*ANCA associated SVV

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