Bone: Difference between revisions

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Mikael Häggström (talk | contribs)
Grossing: Take a small piece
Mikael Häggström (talk | contribs)
Grossing: Separate
 
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*'''Split''' the bone in the plane of interest for microscopy slides.
*'''Split''' the bone in the plane of interest for microscopy slides.
*'''Fix''' the bone in formalin.
*'''Fix''' the bone in formalin.
*Perform '''decalcification''' of the specimen. First, generally take at least a small piece to be kept separately in formalin, in case the main specimen becomes necrotic, so that you have at least one more chance to decalcify it more lightly.  
*Perform '''decalcification''' of the specimen. First, generally take at least a small piece to be kept separately in formalin, in case the main specimen becomes necrotic, so that you have at least one more chance to decalcify it more lightly. If the order and/or history is suspicious for metastasis, try to sample a part of the specimen that is soft enough to not need decalcification (to avoid the risk that decalcification will impair later [[immunohistochemistry]] or other testing).
*Take the '''sections''' of interest.
*Take the '''sections''' of interest.