Immunofluorescense of skin tissues: Difference between revisions
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Suspicion of one or several '''immunologic diseases''':<ref name="ChhabraMinz2012">Page 678 in: {{cite journal|last1=Chhabra|first1=Seema|last2=Minz|first2=RanjanaWalker|last3=Saikia|first3=Biman|title=Immunofluorescence in dermatology|journal=Indian Journal of Dermatology, Venereology, and Leprology|volume=78|issue=6|year=2012|pages=677|issn=0378-6323|doi=10.4103/0378-6323.102355|url=https://pdfs.semanticscholar.org/23d0/148dbe342a93e0bc65a6a2e31040a89113ba.pdf}}</ref> | Suspicion of one or several '''immunologic diseases''':<ref name="ChhabraMinz2012">Page 678 in: {{cite journal|last1=Chhabra|first1=Seema|last2=Minz|first2=RanjanaWalker|last3=Saikia|first3=Biman|title=Immunofluorescence in dermatology|journal=Indian Journal of Dermatology, Venereology, and Leprology|volume=78|issue=6|year=2012|pages=677|issn=0378-6323|doi=10.4103/0378-6323.102355|url=https://pdfs.semanticscholar.org/23d0/148dbe342a93e0bc65a6a2e31040a89113ba.pdf}}</ref> | ||
Suspected conditions where the direct immunofluorescence (DIF) is diagnostic:<ref name="ChhabraMinz2012"/> | Suspected conditions where the '''direct immunofluorescence (DIF) is diagnostic''':<ref name="ChhabraMinz2012"/> | ||
*Bullous diseases | *Bullous diseases | ||
:*Pemphigus (all forms) | :*Pemphigus (all forms) | ||
| Line 17: | Line 17: | ||
:*Systemic lupus erythematosus | :*Systemic lupus erythematosus | ||
Suspected conditions where DIF is highly characteristic and of some value:<ref name="ChhabraMinz2012"/> | ===Highly characteristic=== | ||
Suspected conditions where '''DIF is highly characteristic''' and of some value:<ref name="ChhabraMinz2012"/> | |||
*Vascular diseases | *Vascular diseases | ||
:*Allergic vasculitis | :*Allergic vasculitis | ||
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*Lichen planus | *Lichen planus | ||
Conditions where DIF is not diagnostic but only suggestive of immunologic pathogenesis:<ref name="ChhabraMinz2012"/> | Conditions where '''DIF is not diagnostic but only suggestive''' of immunologic pathogenesis:<ref name="ChhabraMinz2012"/> | ||
*Connective tissue diseases | *Connective tissue diseases | ||
:*Mixed connective tissue diseases | :*Mixed connective tissue diseases | ||
| Line 34: | Line 35: | ||
:*Psoriasis | :*Psoriasis | ||
Suspected conditions where indirect immunofluorescence is generally indicated:<ref name="ChhabraMinz2012"/> | Suspected conditions where '''indirect immunofluorescence is generally indicated''':<ref name="ChhabraMinz2012"/> | ||
*Pemphigus vulgaris | *Pemphigus vulgaris | ||
*Pemphigus foliaceus | *Pemphigus foliaceus | ||
Latest revision as of 05:42, 17 September 2019
Author:
Mikael Häggström [note 1]
Indications
Suspicion of one or several immunologic diseases:[1]
Suspected conditions where the direct immunofluorescence (DIF) is diagnostic:[1]
- Bullous diseases
- Pemphigus (all forms)
- Pemphigoid (all forms)
- Herpes gestationis/gestational pemphigoid
- Dermatitis herpetiformis
- Linear IgA bullous dermatosis Epidermolysis bullosa aquisita
- Connective tissue diseases
- Discoid lupus erythematosus
- Systemic lupus erythematosus
Highly characteristic
Suspected conditions where DIF is highly characteristic and of some value:[1]
- Vascular diseases
- Allergic vasculitis
- Henoch–Schoenlein purpura
- Essential mixed cryoglobulinemia
- Polyarteritis nodosa
- Porphyria
- Especially porphyria cutanea tarda
- Lichen planus
Conditions where DIF is not diagnostic but only suggestive of immunologic pathogenesis:[1]
- Connective tissue diseases
- Mixed connective tissue diseases
- Systemic sclerosis
- Dermatomyositis
- Psoriasis
Suspected conditions where indirect immunofluorescence is generally indicated:[1]
- Pemphigus vulgaris
- Pemphigus foliaceus
- IgA pemphigus
- Bullous pemphigoid
- Dermatitis herpetiformis
- Discoid lupus erythematosus
- Disseminated DLE
- Systemic lupus erythematosus
- Scleroderma
- Mixed connective tissue diseases
- Dermatomyositis
- Vasculitis[notes 1]
- Porphyria[notes 1]
- Lichen planus[notes 1]
- ANCA associated SVV
Fixation
For example Michel's solution,[2] minimally consisting of ammonium sulfate and saline.[3]
Notes
- ↑ For a full list of contributors, see article history. Creators of images are attributed at the image description pages, seen by clicking on the images. See Patholines:Authorship for details.
Main page
References
- ↑ 1.0 1.1 1.2 1.3 1.4 Page 678 in: Chhabra, Seema; Minz, RanjanaWalker; Saikia, Biman (2012). "Immunofluorescence in dermatology ". Indian Journal of Dermatology, Venereology, and Leprology 78 (6): 677. doi:. ISSN 0378-6323. Archived from the original. .
- ↑ Katarzyna Lundmark, Krynitz, Ismini Vassilaki, Lena Mölne, Annika Ternesten Bratel. Handläggning av hudprover – provtagningsanvisningar, utskärningsprinciper och snittning (Handling of skin samples - Instructions for sampling, cutting and incision. KVAST (Swedish Society of Pathology). Retrieved on 2019-09-09.
- ↑ Niedecken, Heinz W.; Lange, Claus-Ekkehard (1985). "Simplified fixative medium for direct immunofluorescence in skin biopsies ". Journal of the American Academy of Dermatology 12 (5): 828–831. doi:. ISSN 01909622.
Image sources