Gross processing: Difference between revisions

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==Cutting==

[[File:Perpendicular versus en face.jpg|thumb|230px|Two major types of sections in gross processing are perpendicular and ''en face'' sections:<br>- '''Perpendicular sections''' allow for measurement of the distance between a lesion and the surgical margin.<br>- '''''En face''''' means that the section is ''tangential'' to the region of interest (such as a lesion) of a specimen. It does not in itself specify whether subsequent microtomy of the slice should be performed on the peripheral or proximal surface of the slice (the peripheral surface of an ''en face'' section is closer to being the ''true'' margin, whereas the proximal surface generally displays more area and therefore generally has greater sensitivity in showing pathology, also compared to perpendicular sections).<br>- A '''shaved''' margin is an ''en face'' margin that contains the entire true margin of the segment.]]

*When cutting with the longer knives, try to cut in '''one stroke''' - do not use like a saw (continuous back and forth)

*Generally, strive to make slices perpendicular to visible '''interfaces''' of relevant tissues.

*Generelly dissect and inspect the '''entire''' specimen, while keeping relevant parts intact enough for presentation to seniors and/or maintaining orientation.

*'''Trim''' tissues for microscopy examination to a thickness of maximum 3-4 mm.<ref group=notes>Thicker slices may not become adequately fixated in formalin.</ref>

===Perpendicular versus en face sections===

[[File:Perpendicular versus en face.jpg|thumb|200px|Perpendicular and ''en face'' sections]]

Two major types of sections in gross processing are perpendicular and ''en face'' sections:

*'''Perpendicular sections''' allow for measurement of the distance between a lesion and the surgical margin.

*'''''En face''''' means that the section is ''tangential'' to the region of interest (such as a lesion) of a specimen. It does not in itself specify whether subsequent microtomy of the slice should be performed on the peripheral or proximal surface of the slice (the peripheral surface of an ''en face'' section is closer to being the ''true'' margin, whereas the proximal surface generally displays more area and therefore generally has greater sensitivity in showing pathology, also compared to perpendicular sections).

*A '''shaved''' margin is an ''en face'' margin that contains the entire true margin of the segment.

==Tissue selection==

@@ Line 22: / Line 22: @@
 ==Cutting==
-[[File:Perpendicular versus en face.jpg|thumb|230px|Two major types of sections in gross processing are perpendicular and ''en face'' sections:<br>- '''Perpendicular sections''' allow for measurement of the distance between a lesion and the surgical margin.<br>- '''''En face''''' means that the section is ''tangential'' to the region of interest (such as a lesion) of a specimen. It does not in itself specify whether subsequent microtomy of the slice should be performed on the peripheral or proximal surface of the slice (the peripheral surface of an ''en face'' section is closer to being the ''true'' margin, whereas the proximal surface generally displays more area and therefore generally has greater sensitivity in showing pathology, also compared to perpendicular sections).<br>- A '''shaved''' margin is an ''en face'' margin that contains the entire true margin of the segment.]]
 *When cutting with the longer knives, try to cut in '''one stroke''' - do not use like a saw (continuous back and forth)
 *Generally, strive to make slices perpendicular to visible '''interfaces''' of relevant tissues.
 *Generelly dissect and inspect the '''entire''' specimen, while keeping relevant parts intact enough for presentation to seniors and/or maintaining orientation.
 *'''Trim''' tissues for microscopy examination to a thickness of maximum 3-4 mm.<ref group=notes>Thicker slices may not become adequately fixated in formalin.</ref>
+===Perpendicular versus en face sections===
+[[File:Perpendicular versus en face.jpg|thumb|200px|Perpendicular and ''en face'' sections]]
+Two major types of sections in gross processing are perpendicular and ''en face'' sections:
+*'''Perpendicular sections''' allow for measurement of the distance between a lesion and the surgical margin.
+*'''''En face''''' means that the section is ''tangential'' to the region of interest (such as a lesion) of a specimen. It does not in itself specify whether subsequent microtomy of the slice should be performed on the peripheral or proximal surface of the slice (the peripheral surface of an ''en face'' section is closer to being the ''true'' margin, whereas the proximal surface generally displays more area and therefore generally has greater sensitivity in showing pathology, also compared to perpendicular sections).
+*A '''shaved''' margin is an ''en face'' margin that contains the entire true margin of the segment.
 ==Tissue selection==

Gross processing: Difference between revisions

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