Bone marrow: Difference between revisions
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→Autopsy: Collection |
→Gross processing: +Insufficient |
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*'''Measure''' length and diameter of each fragment. | *'''Measure''' length and diameter of each fragment. | ||
*If the biopsy is received in a zinc-containing fixative, '''rinse''' it for about 2 minutes, such as placing the cassette in a container under running water (and block any sink enough so that the cassette won't float away). | *If the biopsy is received in a zinc-containing fixative, '''rinse''' it for about 2 minutes, such as placing the cassette in a container under running water (and block any sink enough so that the cassette won't float away). | ||
[[File:Histopathology of bone marrow with insufficient decalcification.jpg|thumb|200px|Histopathology of bone marrow with insufficient decalcification, wherein the bony trabeculae may get pushed by the microtome blade and plow away the cells of interest as displayed. To compensate, thicker sections may be taken, also making evaluation harder.]] | |||
*Put in '''decalcifying''' solution, generally about 15 minutes, and palpate the specimen to check whether it is still firm. If it is, decalcify another 5-10 minutes and check again. Rather have it a little bit too soft than a little bit too firm. Be careful to follow grossing guidelines for bone marrows, since even a small aberration in the processing is likely to be noticed as creating artifacts. | *Put in '''decalcifying''' solution, generally about 15 minutes, and palpate the specimen to check whether it is still firm. If it is, decalcify another 5-10 minutes and check again. Rather have it a little bit too soft than a little bit too firm. Be careful to follow grossing guidelines for bone marrows, since even a small aberration in the processing is likely to be noticed as creating artifacts. | ||