Evaluation: Difference between revisions
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==Microscopy settings== | ==Microscopy settings== | ||
[[File:Light microscopy with and without condenser.jpg|thumb|Light microscopy with and without condenser. At low magnification, using a condenser may limit the field of view, and in such cases it is preferable to not use it. At high magnification, a condenser makes borders less marked, and is generally preferable in such cases.]] | |||
[[File:Calcium pyrophosphate dihydrate crystals without and with condenser, annotated.jpg|thumb|An example of a situation where microscopy without condenser is preferable at high magnification is the evaluation of crystals (calcium pyrophosphate dihydrate crystal deposition disease pictured).]] | |||
Generally the '''condenser''' is placed in its highest position or just slightly lower. At low magnification objectives (mainly 4x and 10x objectives), the opening of the condenser (or iris) diaphragm should be wide open. For high-dry (40x) and oil-immersion objectives (100x), the diaphragm should be closed slowly while looking at a sharply focused section until the level of illumination is just slightly reduced, in order to attain optimal contrast and resolution.<ref>{{cite web|url=https://histologylab.ctl.columbia.edu/HistologyLabManual.pdf|title=Histology Laboratory Manual, Vagelos College of Physicians & Surgeons Columbia University|author=Patrice F Spitalnik|accessdate=2021-09-20}}</ref> | Generally the '''condenser''' is placed in its highest position or just slightly lower. At low magnification objectives (mainly 4x and 10x objectives), the opening of the condenser (or iris) diaphragm should be wide open. For high-dry (40x) and oil-immersion objectives (100x), the diaphragm should be closed slowly while looking at a sharply focused section until the level of illumination is just slightly reduced, in order to attain optimal contrast and resolution.<ref>{{cite web|url=https://histologylab.ctl.columbia.edu/HistologyLabManual.pdf|title=Histology Laboratory Manual, Vagelos College of Physicians & Surgeons Columbia University|author=Patrice F Spitalnik|accessdate=2021-09-20}}</ref> | ||