Immunohistochemistry: Difference between revisions
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==Immunohistochemistry ordering== | ==Immunohistochemistry ordering== | ||
The main approaches to immunohistochemistry ordering are: | The main approaches to immunohistochemistry ordering are: | ||
*Look for a specified '''panel''' for the presentation at hand. For example, for an undifferentiated tumor with no clear lineage differentiation, an initial panel of CK, S100, vimentin and LCA can be used.<ref name="pmid25427040">{{cite journal| author=Lin F, Liu H| title=Immunohistochemistry in undifferentiated neoplasm/tumor of uncertain origin. | journal=Arch Pathol Lab Med | year= 2014 | volume= 138 | issue= 12 | pages= 1583-610 | pmid=25427040 | doi=10.5858/arpa.2014-0061-RA | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=25427040 }} </ref> | *Look for a specified '''panel''' for the presentation at hand. For example, for an undifferentiated tumor with no clear lineage differentiation, an initial panel of cytokeratin (CK), S100, vimentin and LCA (CD45) can be used.<ref name="pmid25427040">{{cite journal| author=Lin F, Liu H| title=Immunohistochemistry in undifferentiated neoplasm/tumor of uncertain origin. | journal=Arch Pathol Lab Med | year= 2014 | volume= 138 | issue= 12 | pages= 1583-610 | pmid=25427040 | doi=10.5858/arpa.2014-0061-RA | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=25427040 }} </ref> | ||
*Coming up with the most relevant '''differential diagnoses''' for the case at hand, and find the immunohistochemistry stains that best distinguish them. Immunohistochemistry profiles for diseases and conditions, as well as their main differential diagnoses, is generally found at '''[https://www.pathologyoutlines.com/ Pathology Outlines]''', or you can pay for a subscription to '''[https://www.immunoquery.com/ ImmunoQuery]'''{{ImmunoQuery COI declaration}}: | *Coming up with the most relevant '''differential diagnoses''' for the case at hand, and find the immunohistochemistry stains that best distinguish them. Immunohistochemistry profiles for diseases and conditions, as well as their main differential diagnoses, is generally found at '''[https://www.pathologyoutlines.com/ Pathology Outlines]''', or you can pay for a subscription to '''[https://www.immunoquery.com/ ImmunoQuery]'''{{ImmunoQuery COI declaration}}: | ||
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GRPR is the top result when specifically comparing lung versus anus squamous cell carcinoma, both at sensitivity settings 1 and 3, with no major difference between them, and thus anus origin is favored in case of either diffuse or focal cytoplasmic staining. DLK showed as N/A for anus SCC. | GRPR is the top result when specifically comparing lung versus anus squamous cell carcinoma, both at sensitivity settings 1 and 3, with no major difference between them, and thus anus origin is favored in case of either diffuse or focal cytoplasmic staining. DLK showed as N/A for anus SCC. | ||
{{Question-end}} | {{Question-end}} | ||
==Immunohistochemistry evaluation== | ==Immunohistochemistry evaluation== | ||
The main methods for evaluating immunohistochemistry results are: | The main methods for evaluating immunohistochemistry results are: | ||