Cervical cytology: Difference between revisions
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File:Lactobacillus sp 01.png|'''Bacterial flora''' (usually rod-shaped ''Lactobacilli''), does not need reporting | File:Lactobacillus sp 01.png|'''Bacterial flora''' (usually rod-shaped ''Lactobacilli''), does not need reporting | ||
File:Micrograph of a clue cell, annotated.jpg|A '''clue cell''', covered in bacteria, as compared to an unremarkable squamous cell at bottom left, and a neutrophil at bottom center. Pap stain. A significant amount of clue cells can be reported as "Shift in vaginal flora suggestive of bacterial vaginosis". | File:Micrograph of a clue cell, annotated.jpg|A '''clue cell''', covered in bacteria, as compared to an unremarkable squamous cell at bottom left, and a neutrophil at bottom center. Pap stain. A significant amount of clue cells can be reported as "Shift in vaginal flora suggestive of bacterial vaginosis". | ||
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File:Cytology of High-Grade SIL, ThinPrep.jpg|High-grade squamous intraepithelial lesion (HSIL) | File:Cytology of High-Grade SIL, ThinPrep.jpg|High-grade squamous intraepithelial lesion (HSIL) | ||
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==Report== | |||
Example in a normal case: | |||
{|class=wikitable | |||
| Cervical/endocervical ThinPrep: | |||
:Negative for intraepithelial lesion or malignancy (NILM). | |||
|} | |||
{{Bottom}} | {{Bottom}} | ||
Revision as of 10:55, 29 August 2022
Author:
Mikael Häggström [note 1]
Adequacy
Adequacy should always be stated, either as "Satisfactory" or "Unsatisfactory". For estimating the number of cells, determine the following:
- The area of your field of view at high power (see the Evaluation chapter)
- The total size of the relevant area on the microscope slide.
- Look at 10 representative high power fields (HPFs) within that area, and calculate the average number of cells per high power field.
| Total number of cells = Average number of cells per HPF * | Total size of area HPF area |
Eventually you will be able to tell when most cases are adequate or inadequate without performing a detailed calculation.
Also state whether the endocervical/transformation zone is present or absent. Count an endocervical component as present if there are 10 or more endocervical or squamous metaplastic cells.[1]
Very common findings
-
Bacterial flora (usually rod-shaped Lactobacilli), does not need reporting
-
A clue cell, covered in bacteria, as compared to an unremarkable squamous cell at bottom left, and a neutrophil at bottom center. Pap stain. A significant amount of clue cells can be reported as "Shift in vaginal flora suggestive of bacterial vaginosis".
Main conditions to exclude or confirm
Squamous atypia, seen mainly as cells with increased nucleus/cytoplasm ratio, nuclear hyperchromasia and irregular nuclear outline.
-
Atypical squamous cells of undetermined significance (ASCUS), with only few slightly atypical cells
-
Low-grade squamous intraepithelial lesion (LSIL)
-
High-grade squamous intraepithelial lesion (HSIL)
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Report
Example in a normal case:
Cervical/endocervical ThinPrep:
|
Notes
- ↑ For a full list of contributors, see article history. Creators of images are attributed at the image description pages, seen by clicking on the images. See Patholines:Authorship for details.
Main page
References
- ↑ Cibas, Edmund S.; Ducatman, Barbara S. (2021). Cytology : diagnostic principles and clinical correlates . Philadelphia, PA. p. 9. ISBN 978-0-323-63637-7. OCLC 1138033641.
Image sources