Evaluation: Difference between revisions

Line 97:

File:Measurement using transparent ruler in microscopy.jpg|You can use a regular transparent ruler for most purposes, since it is rarely necessary to specify distances as more exact than millimeters. It works best on low magnification.

File:Measurement of tumor size on two microscopy slides.jpg|Alternatively, mark the limits under microscopy, and then use a regular ruler to measure the dimensions. This works even if the distance exceeds the field of view, or spans more than one slide.

File:Calibration slide with rulers and grids.jpg|If sub-millimeter measurement is needed, you may use a calibration slide, which is basically a translucent ruler with 0.1 mm line resolution or smaller, which may cost about $15 if none of your colleagues has one to lend, or the department can't provide one for you.

File:Calibration slide with rulers and grids.jpg|Rarely, sub-millimeter measurement is needed, in which case you may use a calibration slide, which is basically a translucent ruler with 0.1 mm line resolution or smaller, which may cost about $15 if none of your colleagues has one to lend, or the department can't provide one for you.

File:Measuring distance on a microscopy slide using a calibration slide.jpg|To measure distance with a calibration slide, put it on top of the tissue slide and switch focus between the two.

</gallery>

There are also eye pieces that show a ruler in the field of view, but make sure you match it with the correct objective and other settings to make the measurement valid.

There are also eye pieces that show a ruler in the field of view, but make sure you match it with the correct objective and other settings to make the measurement valid. A calibration slide is more robust because of such sources of error.

==Counts per mm<sup>2</sup>==

@@ Line 97: / Line 97: @@
 File:Measurement using transparent ruler in microscopy.jpg|You can use a regular transparent ruler for most purposes, since it is rarely necessary to specify distances as more exact than millimeters. It works best on low magnification.
 File:Measurement of tumor size on two microscopy slides.jpg|Alternatively, mark the limits under microscopy, and then use a regular ruler to measure the dimensions. This works even if the distance exceeds the field of view, or spans more than one slide.
-File:Calibration slide with rulers and grids.jpg|If sub-millimeter measurement is needed, you may use a calibration slide, which is basically a translucent ruler with 0.1 mm line resolution or smaller, which may cost about $15 if none of your colleagues has one to lend, or the department can't provide one for you.
+File:Calibration slide with rulers and grids.jpg|Rarely, sub-millimeter measurement is needed, in which case you may use a calibration slide, which is basically a translucent ruler with 0.1 mm line resolution or smaller, which may cost about $15 if none of your colleagues has one to lend, or the department can't provide one for you.
 File:Measuring distance on a microscopy slide using a calibration slide.jpg|To measure distance with a calibration slide, put it on top of the tissue slide and switch focus between the two.
 </gallery>
-There are also eye pieces that show a ruler in the field of view, but make sure you match it with the correct objective and other settings to make the measurement valid.
+There are also eye pieces that show a ruler in the field of view, but make sure you match it with the correct objective and other settings to make the measurement valid. A calibration slide is more robust because of such sources of error.
 ==Counts per mm<sup>2</sup>==

Evaluation: Difference between revisions

Navigation menu

Search