Immunohistochemistry: Difference between revisions
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→Immunohistochemistry ordering: Local routines |
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==Evaluation== | ==Evaluation== | ||
[[File:Positive and negative controls in immunohistochemistry.png|thumb|270px|Confirm that you see the expected results for positive and negative controls. For this purpose, "internal" means tissue from the target patient, and "external" means that the tissue is from another patient.<ref>Image by Mikael Häggström, MD. Reference: {{cite journal| author=Torlakovic EE, Francis G, Garratt J, Gilks B, Hyjek E, Ibrahim M | display-authors=etal| title=Standardization of negative controls in diagnostic immunohistochemistry: recommendations from the international ad hoc expert panel. | journal=Appl Immunohistochem Mol Morphol | year= 2014 | volume= 22 | issue= 4 | pages= 241-52 | pmid=24714041 | doi=10.1097/PAI.0000000000000069 | pmc=4206554 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=24714041 }}</ref>]] | |||
First be familiar with which cells on the slide are being evaluated, such as first looking at a slide with standard staining (usually H&E) to avoid counting background cells. | First be familiar with which cells on the slide are being evaluated, such as first looking at a slide with standard staining (usually H&E) to avoid counting background cells. | ||