Evaluation: Difference between revisions
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Condenser |
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*Look at each microscopy slide by '''eye''', to plan the microscopy screening so as to not miss peripheral fragments. | *Look at each microscopy slide by '''eye''', to plan the microscopy screening so as to not miss peripheral fragments. | ||
*Have a systematic '''direction''' of screening through microscopy slides, such as from top left to bottom right as seen in the microscope. When two-way mirrored, the starting position of the microscope slide is then with the objective pointing at bottom right. | *Have a systematic '''direction''' of screening through microscopy slides, such as from top left to bottom right as seen in the microscope. When two-way mirrored, the starting position of the microscope slide is then with the objective pointing at bottom right. | ||
==Condenser== | |||
Generally the condenser is placed in its highest position or just slightly lower. At low magnification objectives (mainly 4x and 10x objectives), the opening of the condenser (or iris) diaphragm should be wide open. For high-dry (40x) and oil-immersion objectives (100x), the diaphragm should be closed slowly while looking at a sharply focused section until the level of illumination is just slightly reduced, in order to attain optimal contrast and resolution.<ref>{{cite web|url=https://histologylab.ctl.columbia.edu/HistologyLabManual.pdf|title=Histology Laboratory Manual, Vagelos College of Physicians & Surgeons Columbia University|author=Patrice F Spitalnik|accessdate=2021-09-20}}</ref> | |||
==Artifacts== | ==Artifacts== | ||