Immunohistochemistry: Difference between revisions
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→Immunohistochemistry ordering: Upfront |
→Evaluation: +Depending on parts |
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==Evaluation== | ==Evaluation== | ||
First be familiar with which cells on the slide are being evaluated, such as first looking at a slide with standard staining (usually H&E) to avoid counting background cells. | First be familiar with which cells on the slide are being evaluated, such as first looking at a slide with standard staining (usually H&E) to avoid counting background cells. | ||
When the relevant cells take up stains, confirm whether their staining pattern actually counts as positive. For example, a cytoplasmic staining for p63 still counts as "negative (cytoplasmic)" when suspecting squamous, myoepithelial and prostatic basal cells (where only nuclear stain counts as positive). On the other hand, the same cytoplasmic staining for p63 counts as "positive (cytoplasmic)" when suspecting muscle differentiation.<ref name="pmid21623385">{{cite journal| author=Martin SE, Temm CJ, Goheen MP, Ulbright TM, Hattab EM| title=Cytoplasmic p63 immunohistochemistry is a useful marker for muscle differentiation: an immunohistochemical and immunoelectron microscopic study. | journal=Mod Pathol | year= 2011 | volume= 24 | issue= 10 | pages= 1320-6 | pmid=21623385 | doi=10.1038/modpathol.2011.89 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=21623385 }} </ref> | |||
==Interpretation== | ==Interpretation== | ||