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Either before or after a general inspectionFor relatively small amputations (up to trans-metatarsal amputations), fix the body part in formalin, such as either before or after a general inspection. It can be done overnight for digits, and then leaving it for 5-6 hours in a relatively strong '''decalcifying''' solution.
→Gross processing: Expanded
==Gross processing==
*Measure '''length''' and average '''diameter'''
*Determine the anatomic '''location''' of the cut.
*Ink At least for amputated digits, ink the surgical margins differently for soft tissue and bony margin, where samples are to be taken.
'''Sections''' for microscopy are taken as follows:
For amputations resected by cutting across bone:
*Ink For at least amputated digits, ink the bone at the proximal margin, submit perpendicular section of bony margin.
*Skin and soft tissues at proximal margin.
:*If margin is close to gangrene- perpendicular sections
===Gross report===
Examplefor digits:
{|class=wikitable
| {{Moderate-begin}}A. Labeled - ___. The specimen is received in formalin and consists of an amputated __.{{Moderate-end}} It measures ___ cm in length and ___ cm in average diameter. It is resected ___ {{Comment-begin}}location{{Comment-end}}. {{Finding-begin}}The proximal ___ cm of the specimen is not covered by skin and soft tissue.{{Finding-end}} The skin and soft tissue margins appear <viable / necrotic>. The skin surface appears ___ {{Finding-begin}}and displays an (ulcerated/necrotic/gangrenous) lesion, cm from the cutaneous margin{{Finding-end}}. The nail(s) is/are <color/thickened/absent/necrotic>. The soft tissue surgical margin is inked blue {{Comment-begin}}for example{{Comment-end}}, and the bony surgical margin of the ___ {{Comment-begin}}specific bone involved{{Comment-end}} is inked green {{Comment-begin}}for example{{Comment-end}}. On cut sections, the bone subjacent to the ulcer shows no gross abnormalities. Representative sections are submitted for microscopic examination in ___ cassettes following decalcification.
