Difference between revisions of "Bone"

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(→‎Grossing: Separate)
 
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<noinclude>{{Top
 
|author1=[[User:Mikael Häggström|Mikael Häggström]]
 
|author1=[[User:Mikael Häggström|Mikael Häggström]]
 
|author2=
 
|author2=
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*[[Toe]]
 
*[[Toe]]
 
*[[Avascular necrosis]]
 
*[[Avascular necrosis]]
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*'''Bone tumors''': Generally correlate with age, location and radiology.
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</noinclude>
  
 
==Grossing==
 
==Grossing==
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*'''Split''' the bone in the plane of interest for microscopy slides.
 
*'''Split''' the bone in the plane of interest for microscopy slides.
 
*'''Fix''' the bone in formalin.
 
*'''Fix''' the bone in formalin.
*Perform '''decalcification''' of the specimen
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*Perform '''decalcification''' of the specimen. First, generally take at least a small piece to be kept separately in formalin, in case the main specimen becomes necrotic, so that you have at least one more chance to decalcify it more lightly. If the order and/or history is suspicious for metastasis, try to sample a part of the specimen that is soft enough to not need decalcification (to avoid the risk that decalcification will impair later [[immunohistochemistry]] or other testing).
 
*Take the '''sections''' of interest.
 
*Take the '''sections''' of interest.
  
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File:Histopathology of acute osteomyelitis.jpg|Acute '''[[osteomyelitis]]''': Numerous neutrophils in the stroma.
 
File:Histopathology of acute osteomyelitis.jpg|Acute '''[[osteomyelitis]]''': Numerous neutrophils in the stroma.
 
</gallery>
 
</gallery>
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===Metastasis===
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Highly suspicious [[prostate adenocarcinoma]] metastasis can be confirmed with NKX1, TTF1 and CDX2.
 
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Latest revision as of 11:33, 20 November 2023

Author: Mikael Häggström [note 1]

Subregions

Presentations


Grossing

To submit slides for microscopy, generally gross as follows:

  • Split the bone in the plane of interest for microscopy slides.
  • Fix the bone in formalin.
  • Perform decalcification of the specimen. First, generally take at least a small piece to be kept separately in formalin, in case the main specimen becomes necrotic, so that you have at least one more chance to decalcify it more lightly. If the order and/or history is suspicious for metastasis, try to sample a part of the specimen that is soft enough to not need decalcification (to avoid the risk that decalcification will impair later immunohistochemistry or other testing).
  • Take the sections of interest.

Microscopic evaluation

Look for any of the following:

Metastasis

Highly suspicious prostate adenocarcinoma metastasis can be confirmed with NKX1, TTF1 and CDX2.

Notes

  1. For a full list of contributors, see article history. Creators of images are attributed at the image description pages, seen by clicking on the images. See Patholines:Authorship for details.

Main page

References


Image sources