Difference between revisions of "Bone"
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(→Presentations: Correlate) Tags: Mobile web edit, Mobile edit |
(→Grossing: Take a small piece) |
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*'''Split''' the bone in the plane of interest for microscopy slides. | *'''Split''' the bone in the plane of interest for microscopy slides. | ||
*'''Fix''' the bone in formalin. | *'''Fix''' the bone in formalin. | ||
| − | *Perform '''decalcification''' of the specimen | + | *Perform '''decalcification''' of the specimen. First, generally take at least a small piece to be kept separately in formalin, in case the main specimen becomes necrotic, so that you have at least one more chance to decalcify it more lightly. |
*Take the '''sections''' of interest. | *Take the '''sections''' of interest. | ||
Revision as of 13:44, 2 January 2023
Author:
Mikael Häggström [note 1]
Contents
Subregions
Presentations
- Osteomyelitis
- Toe
- Avascular necrosis
- Bone tumors: Generally correlate with age, location and radiology.
Grossing
To submit slides for microscopy, generally gross as follows:
- Split the bone in the plane of interest for microscopy slides.
- Fix the bone in formalin.
- Perform decalcification of the specimen. First, generally take at least a small piece to be kept separately in formalin, in case the main specimen becomes necrotic, so that you have at least one more chance to decalcify it more lightly.
- Take the sections of interest.
Microscopic evaluation
Look for any of the following:
Acute osteomyelitis: Numerous neutrophils in the stroma.
Metastasis
Highly suspicious prostate adenocarcinoma metastasis can be confirmed with NKX1, TTF1 and CDX2.
Notes
- ↑ For a full list of contributors, see article history. Creators of images are attributed at the image description pages, seen by clicking on the images. See Patholines:Authorship for details.
Main page
References
Image sources
