Difference between revisions of "Bone marrow"
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| '''Bone marrow''' from rib (or other location if applicable): Trilineage hematopoiesis. There is no evidence of malignancy. | | '''Bone marrow''' from rib (or other location if applicable): Trilineage hematopoiesis. There is no evidence of malignancy. | ||
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| + | ==Bone marrow aspirate== | ||
| + | Perform a '''differential count''' by counting 500 cells<ref name="pmid29757362">{{cite journal| author=Abdulrahman AA, Patel KH, Yang T, Koch DD, Sivers SM, Smith GH | display-authors=etal| title=Is a 500-Cell Count Necessary for Bone Marrow Differentials?: A Proposed Analytical Method for Validating a Lower Cutoff. | journal=Am J Clin Pathol | year= 2018 | volume= 150 | issue= 1 | pages= 84-91 | pmid=29757362 | doi=10.1093/ajcp/aqy034 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=29757362 }} </ref> and dividing the numbers by 5 to get their percentages. Don't count cells that don't have a cytoplasm or nucleus. | ||
==Bone marrow biopsy== | ==Bone marrow biopsy== | ||
Revision as of 14:50, 16 December 2021
Author:
Mikael Häggström [note 1]
Contents
Autopsy
Microscopic evaluation
- Confirm trilineage hematopoiesis (see image).
- Look for apparent cellular atypia or decrease of cellular diversity.
Report
Example in a normal case:
| Bone marrow from rib (or other location if applicable): Trilineage hematopoiesis. There is no evidence of malignancy. |
Bone marrow aspirate
Perform a differential count by counting 500 cells[1] and dividing the numbers by 5 to get their percentages. Don't count cells that don't have a cytoplasm or nucleus.
Bone marrow biopsy
Evaluate the following:
- Adequacy: There should preferably be 5 intertrabecular spaces.
- Cellularity: In patients 20-80 years, the percentage should be about 100 minus age, such as 40% in a 60 year old patient.
- Thrombopoiesis: There are normally about 3 megakaryocytes per 40x field.
- Myeloid/erythroid ratio, normally 3-4 myeloid cells per erythroid cell.
- Look for any granuloma or cancer metastasis
In a bone marrow count, nucleated red blood cells count into the total percentage of cells (but does not count into percentage of white blood cells in peripheral blood).
Notes
- ↑ For a full list of contributors, see article history. Creators of images are attributed at the image description pages, seen by clicking on the images. See Patholines:Authorship for details.
Main page
References
- ↑ Abdulrahman AA, Patel KH, Yang T, Koch DD, Sivers SM, Smith GH (2018). "Is a 500-Cell Count Necessary for Bone Marrow Differentials?: A Proposed Analytical Method for Validating a Lower Cutoff. ". Am J Clin Pathol 150 (1): 84-91. doi:. PMID 29757362. Archived from the original. .
Image sources
