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→Gross processing: +Example
Breast specimens should be immersed for 6-72 hours.<ref>{{cite web|url=https://webapps.cap.org/apps/docs/committees/immunohistochemistry/her2_faqs.pdf|title=Recommendations for HER2 Testing in Breast Cancer: ASCO – CAP Clinical Practice Guideline Update|date=2013-10-17|website=College of American Pathologists}}</ref>
{{Comprehensiveness|otherlegend=yes}}
==Gross processing==
===Selection and trimming===
For excision (also called lumpectomy):*Determine total specimen '''size'''. Optionally, determine weight{{Comprehensive-begin}}Weight the specimen<ref name=Roychowdhury>{{cite web|url=https://www.pathologyoutlines.com/topic/breastmalignantgrossing.html|title=Grossing (histologic sampling) of breast lesions|author=Monika Roychowdhury|website=Pathologyoutlines.com}} Topic Completed: 1 August 2012. Revised: 19 September 2019</ref>{{Comprehensive-end}}
*'''Ink''' margins.{{Ink note}} If sample orientations are marked, use different colors for different directions.<ref name=Roychowdhury/>
*'''Palpate''' the specimen for masses. Compare with radiograph if available<ref name=Roychowdhury/>
*Make 3-4 mm thick '''slices'''.<ref name=Roychowdhury/>
*Submit:<ref name=Roychowdhury/>
{{Gross processing}}
===ReportGross report===
*Size of original tissue '''sample''', preferably in 3 dimensions.
*Description of inking
*Tumor properties, at least:
:*Size in 3 dimensions.<ref name=Roychowdhury/>
:*Distance from margins<ref name=Roychowdhury/>
:*Consistency<ref name=Roychowdhury/>
*{{Moderate-begin}}Description of sectioning and submissions.{{Moderate-end}}
*{{Comprehensive-begin}}Time of procurement and time of placement in formalin, in order to be able to calculate the cold ischemia time, which has effects on immunohistochemistry.<ref name="Khoury2018">{{cite journal|last1=Khoury|first1=Thaer|title=Delay to Formalin Fixation (Cold Ischemia Time) Effect on Breast Cancer Molecules|journal=American Journal of Clinical Pathology|volume=149|issue=4|year=2018|pages=275–292|issn=0002-9173|doi=10.1093/ajcp/aqx164}}</ref>{{Comprehensive-end}}
Example:
{|class=wikitable
| {{Moderate-begin}}The specimen is received fresh and consists of{{Moderate-end}} an irregular fragment of yellow tan fibrofatty tissue measuring 4.0 x 2.8 x 2.0 cm. {{Moderate-begin}}The specimen is oriented with two sutures and{{Moderate-end}} the surgical margins are inked as follows: superior-blue, inferior-green, medial-red, lateral-yellow, anterior-orange, and posterior-black. {{Finding-begin}}A radioactive seed is embedded in the tissue.{{Finding-end}} The tissue is serially sectioned {{Finding-begin}}to reveal a tan-gray, spiculated, indurated mass measuring _cm in greatest dimension. The mass is located _cm from the nearest (<<superior, inferior etc>>) margin. {{Moderate-begin}}The specimen is entirely submitted in sequential sections from medial to lateral in 10 cassettes. The medial and lateral margins are submitted as perpendicular sections.{{Moderate-end}} The specimen was procured at _AM/PM on _/_/2020. The specimen was placed in formalin at _AM/PM on _/_/2020.
|}
==Staining==
