Difference between revisions of "Evaluation"
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*First, look at each microscopy slide by '''eye''', to plan the microscopy screening so as to not miss peripheral fragments. | *First, look at each microscopy slide by '''eye''', to plan the microscopy screening so as to not miss peripheral fragments. | ||
*Have a systematic '''direction''' of screening through microscopy slides, such as from top left to bottom right as seen in the microscope. When two-way mirrored, the starting position of the microscope slide is then with the objective pointing at bottom right. | *Have a systematic '''direction''' of screening through microscopy slides, such as from top left to bottom right as seen in the microscope. When two-way mirrored, the starting position of the microscope slide is then with the objective pointing at bottom right. | ||
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| + | ==Artifacts== | ||
| + | In microscopy, an artifact is an apparent structural detail that is caused by the processing of the specimen and is thus not a legitimate feature of the specimen. Major artifacts to account for include: | ||
| + | <gallery mode=packed> | ||
| + | File:Cellulose contamination in H&E and polarized light.jpg|Cellulose '''contamination''', here seen on H&E stain and polarized light, respectively. | ||
| + | File:Histopathology of cardiac muscle with contamination from thyroid tissue.jpg|Cardiac muscle (bottom) with '''contamination''' from thyroid tissue (center). | ||
| + | File:Crush artifact from forceps.jpg|'''Crush''' artifact from compression by forceps on the tissue sample. | ||
| + | File:Skin with folds and crush artifact by needle.jpg|'''Folding''' artifacts (white arrows) and a crush artifact (black arrow, with [[cytoplasmic hypereosinophilia]] and [[nuclear pleomorphism]]) from a needle. | ||
| + | File:Tearing artifacts in histopathology.jpg|'''Tearing''' artifacts, such as can be caused by:<br>- Microtomy with a nick or blemish in the knife edge.<ref name="TaqiSami2018">{{cite journal|last1=Taqi|first1=SyedAhmed|last2=Sami|first2=SyedAbdus|last3=Sami|first3=LateefBegum|last4=Zaki|first4=SyedAhmed|title=A review of artifacts in histopathology|journal=Journal of Oral and Maxillofacial Pathology|volume=22|issue=2|year=2018|pages=279|issn=0973-029X|doi=10.4103/jomfp.JOMFP_125_15}}</ref><br>- Traction of the sections.<br>- Too much or too little alcohol dehydration.<ref name="TaqiSami2018"/><br>- Sectioning calcified parts, which can be decalcified or removed.<ref name="TaqiSami2018"/> | ||
| + | File:Microscopy of liver parenchyma with tearing artifacts.jpg|More '''tearing''' artifacts, showing that they may be more circular than fusiform. | ||
| + | File:Formalin pigment artifacts.jpg|''''Formalin pigment''' artifacts | ||
| + | File:Air bubble entrapment artifacts.jpg|'''Air bubble entrapment''' artifacts | ||
| + | File:Staining artifacts by residual wax.jpg|'''Staining''' artifacts by residual wax, resulting in pale areas where cellular structures are not discernible. | ||
| + | File:Histopathology of radically excised basal-cell carcinoma with separation artifact (horizontal layout).jpg|A '''separation''' artifact in top image makes the tumor look incompletely excised, but the next microtomy level (bottom image) shows a surgical margin of connective tissue. | ||
| + | </gallery> | ||
{{Bottom}} | {{Bottom}} | ||
Revision as of 15:26, 16 July 2021
Author:
Mikael Häggström [note 1]
.jpg)
- First, look at each microscopy slide by eye, to plan the microscopy screening so as to not miss peripheral fragments.
- Have a systematic direction of screening through microscopy slides, such as from top left to bottom right as seen in the microscope. When two-way mirrored, the starting position of the microscope slide is then with the objective pointing at bottom right.
Artifacts
In microscopy, an artifact is an apparent structural detail that is caused by the processing of the specimen and is thus not a legitimate feature of the specimen. Major artifacts to account for include:
Cellulose contamination, here seen on H&E stain and polarized light, respectively.
Cardiac muscle (bottom) with contamination from thyroid tissue (center).
Crush artifact from compression by forceps on the tissue sample.
Folding artifacts (white arrows) and a crush artifact (black arrow, with cytoplasmic hypereosinophilia and nuclear pleomorphism) from a needle.
More tearing artifacts, showing that they may be more circular than fusiform.
'Formalin pigment artifacts
Air bubble entrapment artifacts
Staining artifacts by residual wax, resulting in pale areas where cellular structures are not discernible.
A separation artifact in top image makes the tumor look incompletely excised, but the next microtomy level (bottom image) shows a surgical margin of connective tissue.
.jpg)
Notes
- ↑ For a full list of contributors, see article history. Creators of images are attributed at the image description pages, seen by clicking on the images. See Patholines:Authorship for details.
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