Difference between revisions of "Pathology for clinicians and medical students"

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Important points for everyone who may send specimens to pathology:
 
Important points for everyone who may send specimens to pathology:
*Generally put specimens in '''formalin'''
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:*When sending a [[tophus]] or other specimen suspicious for gout versus pseudogout, send the specimen in alcohol or dry, since formalin will dissolve the crystals.  
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==Fixation==
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<!--Also in Fixation article
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-->Within an hour after removal from the body,<ref>{{cite web|url=https://www.uclahealth.org/pathology/workfiles/Education/Residency%20Program/Gross%20Manual/Mastectomy%2006.03.20.pdf|title=Breast pathology grossing guidelines|website=UCLA Health|accessdate=2021-09-09}}</ref> tissue samples should generally be placed in vessels with the correct fixing solution, with a volume that allows them to lie freely in the solution.<ref name=kvast>{{cite web|url=http://www.svfp.se/foreningar/uploads/L15178/kvast/hud/Handlaggning%20av%20hudprover%20%20provtagningsanvisningar%20utskarningsprinciper%20och%20snittning%2020150325.pdf|title=Handläggning av hudprover – provtagningsanvisningar, utskärningsprinciper och snittning (Handling of skin samples - Instructions for sampling, cutting and incision|author=Katarzyna Lundmark, Krynitz, Ismini Vassilaki, Lena Mölne, Annika Ternesten Bratel|accessdate=2019-09-09|website=KVAST (Swedish Society of Pathology)}}</ref> The standard fixation fluid is generally 10% neutral buffered '''formalin''', which is roughly equivalent to 4% formaldehyde.<ref>{{cite web|url=https://microscopy.duke.edu/guides/paraformaldehyde-formaldehyde-formalin|title=Paraformaldehyde, Formadehyde and Formalin|website=Duke University|accessdate=2019-12-17}}</ref>
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The main exception to using formalin are mainly:
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*A '''[[tophus]]''' or other specimen suspicious for gout versus pseudogout should be sent in alcohol or dry, since formalin will dissolve the crystals.
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*[[Lymph node]]s (or other lymphoid aggregates) with a suspicion of '''lymphoma''', where samples are generally put in a special solution for flow cytometry.
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*[[Products of conception]] with a need to take samples for '''genetic testing'''.
 
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Revision as of 18:11, 10 September 2021

Author: Mikael Häggström [note 1]
Important points for everyone who may send specimens to pathology:

Fixation

Within an hour after removal from the body,[1] tissue samples should generally be placed in vessels with the correct fixing solution, with a volume that allows them to lie freely in the solution.[2] The standard fixation fluid is generally 10% neutral buffered formalin, which is roughly equivalent to 4% formaldehyde.[3]

The main exception to using formalin are mainly:

  • A tophus or other specimen suspicious for gout versus pseudogout should be sent in alcohol or dry, since formalin will dissolve the crystals.
  • Lymph nodes (or other lymphoid aggregates) with a suspicion of lymphoma, where samples are generally put in a special solution for flow cytometry.
  • Products of conception with a need to take samples for genetic testing.

Notes

  1. For a full list of contributors, see article history. Creators of images are attributed at the image description pages, seen by clicking on the images. See Patholines:Authorship for details.

Main page

References

  1. . Breast pathology grossing guidelines. UCLA Health. Retrieved on 2021-09-09.
  2. Katarzyna Lundmark, Krynitz, Ismini Vassilaki, Lena Mölne, Annika Ternesten Bratel. Handläggning av hudprover – provtagningsanvisningar, utskärningsprinciper och snittning (Handling of skin samples - Instructions for sampling, cutting and incision. KVAST (Swedish Society of Pathology). Retrieved on 2019-09-09.
  3. . Paraformaldehyde, Formadehyde and Formalin. Duke University. Retrieved on 2019-12-17.

Image sources