Template:Immunohistochemistry evaluation of invasive breast cancer

Immunohistochemistry

• Ki-67 index:

Ki-67 in an invasive breast cancer: cancer nuclei are stained (brown). There is tumor cell positivity in 70% of the cells (Ki-67 labelling index = 70%).

• At least three high-power (×40 objective) fields should be selected
• At least 500 malignant invasive cells should be counted, unless a protocol clearly states reasons for fewer being acceptable.
• The invasive edge of the tumor should be scored
• If a comparisons must be made between core biopsies and sections from an excision, evaluation of the latter should be across the whole tumor.
• Only nuclear staining counts. Staining intensity of a positive nucleus is not relevant.

• HER2/neu score or status:

Score	Status
0 to 1+	HER2 negative (not present)
2+	Borderline
3+	HER2 positive

• 

  HER2/neu staining is seen as a cell membrane with continuous brown color.

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