Template:Immunohistochemistry evaluation of invasive breast cancer
Contents
Immunohistochemistry
Ki-67 index
For Ki-67 index, there are two main methods, a faster "hot spot" method and the more comprehensive IKWG global average:
- Hot spot:
Hot spots are areas in which Ki-67 staining is particularly higher relative to the adjacent tumor areas.[1] Usually, the invasive edge of a tumor is a hot spot.[1] When a tumor had several hot spots, the “hottest” spot is selected.[1] Aim to count at least 500 cells in each case, but this is not always possible in cases with low tumor cell density and small tumor size.[1] Also aim to include at least three high-power (×40 objective) fields.[2]
- IKWG global average:[3]
- Before first use, access the IKWG website (https://www.ki67inbreastcancerwg.org/) and complete the Ki67 calibration exercise
- From Tools, link to the Online scoring app (or download and install the Ki67 counting app) and use the global method
- Using a regular light microscope, review the Ki67-stained breast cancer slide and input estimates of the percent area with negligible, low, medium, or high Ki67 index
- Score 100 nuclei negative or positive in each field type (as directed by the app)
- Record “Weighted global score” output as the Ki67 index for that slide
Regardless of method:[2]
- If a comparisons must be made between core biopsies and sections from an excision, evaluation of the latter should be across the whole tumor.
- Only nuclear staining counts. Staining intensity of a positive nucleus is not relevant.
HER2/neu
Score[4] | Status[4] |
---|---|
0 to 1+ | HER2 negative (not present) |
2+ | Borderline |
3+ | HER2 positive |
Notes
Main page
References
- ↑ 1.0 1.1 1.2 1.3 Coleman, William B.; Jang, Min Hye; Kim, Hyun Jung; Chung, Yul Ri; Lee, Yangkyu; Park, So Yeon (2017). "A comparison of Ki-67 counting methods in luminal Breast Cancer: The Average Method vs. the Hot Spot Method ". PLOS ONE 12 (2): e0172031. doi: . ISSN 1932-6203.
- ↑ 2.0 2.1 Dowsett, M.; Nielsen, T. O.; A'Hern, R.; Bartlett, J.; Coombes, R. C.; Cuzick, J.; Ellis, M.; Henry, N. L.; et al. (2011). "Assessment of Ki67 in Breast Cancer: Recommendations from the International Ki67 in Breast Cancer Working Group ". JNCI Journal of the National Cancer Institute 103 (22): 1656–1664. doi: . ISSN 0027-8874.
- ↑ Nielsen, Torsten O; Leung, Samuel C. Y; Rimm, David L; Dodson, Andrew; Acs, Balazs; Badve, Sunil; Denkert, Carsten; Ellis, Matthew J; et al. (2021). "Assessment of Ki67 in Breast Cancer: Updated Recommendations From the International Ki67 in Breast Cancer Working Group ". JNCI: Journal of the National Cancer Institute 113 (7): 808–819. doi: . ISSN 0027-8874.
- ↑ 4.0 4.1 . IHC Tests (ImmunoHistoChemistry). Breastcancer.org. Retrieved on 2019-10-04. Last modified on October 23, 2015
Image sources