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- Weigh the specimen.
- The size of the testis in 3 dimensions
- The spermatic cord length and diameter
- Ink the tunica vaginalis.
- Cut through tunica vaginalis with scissors
- Bisect the testis in the plane of the epididymis.
- Photograph the cut surface.
- If any tumor:
- Measure the size of the tumor in 3 dimensions
- Describe the gross appearance.
- Measure the distance of the tumor from the tunica vaginalis
- Note tumor extension: up to, into, or through the tunica albuginea or vaginalis.
- Measure the distance of the tumor from the epididymis and spermatic cord, and note any epididymal and spermatic cord involvement.
- Describe the remaining testicular parenchyma.
- If the specimen is large, serially section perpendicular to the long axis.
- Fix in formalin overnight.
Generally including the following:
- The proximal spermatic cord margin en face[notes 2].
- For any tumor:
- At least 1 section per cm of tumor, including the closest penetration of tunica albuginea/vaginalis and epididymis.
- Grossly different areas of tumor to determine which components are present (in case of potential mixed germ cell tumor).
- Additional sections of the spermatic cord
- Normal (uninvolved) testis.
- Any found lymph nodes
In case of orchiectomy for undescended testis, submit the entire testicular parenchyma to evaluate for GCNIS (germ cell neoplasia in situ).
- For a full list of contributors, see article history. Creators of images are attributed at the image description pages, seen by clicking on the images. See Patholines:Authorship for details.
- En face means that the section is tangential to the region of interest (such as a lesion) of a specimen. It does not in itself specify whether microtomy of the slice should be performed on the peripheral or proximal surface of the slice.
- Nicole Cipriani. Testis. The University of Chicago Department of Pathology. Retrieved on 2021-03-31.