Template:Epidermal and junctional nevus grading

((Grade a nevus into either low or high grade by the following algorithm:))

File:Algorithm for low grade versus high grade dysplastic nevus.png

In uncertain cases, more specific grading, and/or differentiation from a non-atypical congenital nevus and suspected melanoma, the following table can be used to determine the most fitting diagnosis:

Comparison of congenital pattern nevus, dysplastic nevus and suspected melanoma  

Parameter		Non-atypical congenital pattern	Low-grade dysplastic nevus		High-grade dysplastic nevus	Suspected melanoma in situ
			Mild dysplasia	Moderate dysplasia	Severe dysplasia
Macroscopic	Lateral circumscription	Sharp	Slightly diminished	Moderate		Poor File:Excised melanoma in situ.jpg
	Symmetry	Good			Often broken	Rare
Structural (Low mag.)	Delimitation		Rarely diffuse	Sometimes diffuse	Often diffuse
	Lentiginous proliferation		Yes, along with rete pegs	Yes, along with and focally between rete pegs	Yes, along with and focally between rete pegs	Yes partially continuous, multilayered File:Histopathology of lentigo maligna.jpg
	Bridging		Rarely	Often
	Confluent nests		Rarely	Sometimes	Often	Often widespread
	Pigment distribution		Regular	Irregular
	Suprabasal presence (less than most superficial third of subcorneal epidermis)	Occasionally centrally	No or rarely	Occasionally centrally		Yes, multifocal
	Pagetoid migration including superficial third of subcorneal epidermis		No	No	Yes, in a maximum of 2 HPF centrally, but not peripherally	Yes, multifocal and/or in periphery File:Pagetoid peripherally to a melanoma in situ (crop).jpg Peripheral.
	Extended rete pegs	Ocassional	Yes, regular	Yes, varying	Yes, often irregular	Varying, flattened
	Concentric fibrosis	Regressive	Yes			Occasional
	Lamellar fibrosis		Rarely	Often	Often pronounced	Occasional
	Lymphocytic infiltrate		Mild, perivascular	Mild or moderate, perivascular	Varying	Varying
	Suprapapillary plate involvement	No		Usually no	Often	Yes
Cellular (high mag.)	Image			File:Compound nevus with moderate atypia.jpg
	Junctional extension	Unusual	Usual			Extensive
	Nuclear size	Age-related	Small	Medium	Large	Medium or large. Pleomorphic
	Nuclear pleomorphism		Slight	Prominent
	Chromatin pattern	Uniform	Condensed	Partically expanded	Expanded, coarse in some cells	Expanded, hyperchromatic, coarse. Usually granular.
	Nucleoli	Age-related	Small	Medium	Large	Usually large
	Mitoses	Few superficial				Superficial and deep
	Histological regression		Usually		Usually not
	Percentage of atypical melanocytes		<10%	About 10 - 50%	about 50-90%	Usually> 90%
	Intradermal melanocytic atypia		No	Rarely, in superficial part	Can be detected in superficial part
	Intradermal melanocyte maturation		Yes	Yes, can be partial	Yes, can be partial	Variable

In suspected but not certain nevus or melanoma in situ, generally perform immunohistochemistry with SOX10, whereby melanocyte proliferation and nuclear pleomorphism is easier to see.

• SOX10 immunohistochemistry of a junctional nevus, with atypical melanocytic proliferation, seen mainly in hair follicles.
  SOX10 immunohistochemistry of a junctional nevus, with atypical melanocytic proliferation, seen mainly in hair follicles.
• SOX10 immunohistochemistry of lentigo maligna, showing an increased number of melanocytes along stratum basale, and nuclear pleumorphism.
  SOX10 immunohistochemistry of lentigo maligna, showing an increased number of melanocytes along stratum basale, and nuclear pleumorphism.