Bone marrow: Difference between revisions

From patholines.org
Jump to navigation Jump to search
Mikael Häggström (talk | contribs)
Cells
Mikael Häggström (talk | contribs)
+Flow
Line 27: Line 27:


In a bone marrow count, '''nucleated red blood cells''' count into the total percentage of cells (but does not count into percentage of white blood cells in peripheral blood).
In a bone marrow count, '''nucleated red blood cells''' count into the total percentage of cells (but does not count into percentage of white blood cells in peripheral blood).
==See also==
*[[Flow cytometry]]
{{Bottom}}
{{Bottom}}

Revision as of 13:50, 4 January 2022

Author: Mikael Häggström [note 1]

File:Trilineage hematopoiesis, annotated.jpg
Bone marrow aspirate showing normal "trilineage hematopoiesis":
- Myelomonocytic cells: an eosinophil myelocyte marked
- Erythroid cells: an orthochromatic erythroblast marked
- Megakaryocytic cells.

Autopsy

Microscopic evaluation

  • Confirm trilineage hematopoiesis (see image).
  • Look for apparent cellular atypia or decrease of cellular diversity.

Report

Example in a normal case:

Bone marrow from rib (or other location if applicable): Trilineage hematopoiesis. There is no evidence of malignancy.

Bone marrow aspirate

Perform a differential count by counting 500 cells[1] and dividing the numbers by 5 to get their percentages. Don't count cells that don't have a cytoplasm or nucleus.

Bone marrow biopsy

Evaluate the following:

  • Adequacy: There should preferably be 5 intertrabecular spaces.
  • Cellularity: In patients 20-80 years, the percentage should be about 100 minus age, such as 40% in a 60 year old patient.
  • Thrombopoiesis: There are normally about 3 megakaryocytes per 40x field.
  • Myeloid/erythroid ratio, normally 3-4 myeloid cells per erythroid cell.
  • Look for any granuloma or cancer metastasis

In a bone marrow count, nucleated red blood cells count into the total percentage of cells (but does not count into percentage of white blood cells in peripheral blood).

See also

Notes

  1. For a full list of contributors, see article history. Creators of images are attributed at the image description pages, seen by clicking on the images. See Patholines:Authorship for details.

Main page

References

  1. Abdulrahman AA, Patel KH, Yang T, Koch DD, Sivers SM, Smith GH (2018). "Is a 500-Cell Count Necessary for Bone Marrow Differentials?: A Proposed Analytical Method for Validating a Lower Cutoff. ". Am J Clin Pathol 150 (1): 84-91. doi:10.1093/ajcp/aqy034. PMID 29757362. Archived from the original. . 

Image sources